164,304 results

1. pYFAC-CH2-ubi-M-pyrG

   Plasmid

   #221132

   Purpose

   Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

   Type

   Empty backbone

   Use

   Synthetic Biology; Expression in aspergillus and …

   Tags

   Expression

   Mutation

   Promoter

   PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBR-Cas9ΔyqhD-HA500

   Plasmid

   #220369

   Purpose

   A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

   Depositor

   Yilan Liu

   Inserts

   SpCas9
   sgRNA for yqhD in P. putida KT2440
   

   Use

   CRISPR; Broad host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR2.1 Clover-LMNA Donor

   Plasmid

   #122508

   Purpose

   Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
   

   Use

   Homology repair template plasmid (donor plasmid)

   Tags

   Clover

   Expression

   Mutation

   Promoter

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-ChrimsonR-mCherry

   Plasmid

   #92207

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   ChimsonR-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   June 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKOV

   Plasmid

   #25769

   Depositor

   George Church

   Article

   Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. iF59 PB-TO-oNgn2-puro

   Plasmid

   #204734

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Optimized neurogenin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pimEJ5GFP

   Plasmid

   #44026

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ5GFP egfp-based chromosomal break reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pCAGGS promoter and eGFP separated by pgkPURO cas…

   Promoter

   pCAGGS

   Available Since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR GFP-CaaX

   Plasmid

   #113020

   Purpose

   for lentivirus production of GFP-CaaX

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   GFP-CaaX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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