We narrowed to 88,318 results for: Mal

1. pcDNA3. Flag-CSL R192H

   Plasmid

   #167005

   Purpose

   Mammalian expression of CSL R192H

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R192H

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

   Plasmid

   #64220

   Purpose

   Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

   Plasmid

   #64217

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   hU6 promoter; BbsI sites for sgRNA
   H1 promoter; BamHI site for shRNA
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Promoter

   CBh, H1, and U6

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Circular 200,100 with interspersed loops (GAPDH)

   Plasmid

   #180183

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF CAG BFP2 IRES neo

   Plasmid

   #108175

   Purpose

   Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Blue fluorescent protein (BFP2)
   

   Use

   Lentiviral; Fluorescent protein

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro-2xMyc-Rac1-P29S

   Plasmid

   #128581

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine.

   Available Since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

   Plasmid

   #97044

   Purpose

   Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-N2H-N1

   Plasmid

   #125547

   Purpose

   Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 attached to n-terminus of Gatew…

   Expression

   Bacterial and Mammalian

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-N2H-C1

   Plasmid

   #125549

   Purpose

   Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 attached to c-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-N2H-C2

    Plasmid

    #125559

    Purpose

    Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to c-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLgw Cbx1-V5-EcoDam

    Plasmid

    #59211

    Purpose

    Mammalian DamID positive control lentiviral plasmid. Expresses Cbx1-V5-Dam.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter and heat shock minima…

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-N2H-N2

    Plasmid

    #125548

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 attached to n-terminus of Gatew…

    Expression

    Mammalian and Yeast

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. cacnb4 (rat) in pMT2 vector

    Plasmid

    #107426

    Purpose

    Expresses Calcium channel beta4 auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

    Insert

    cacnb4 (Cacnb4 Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-Flag-FBW7K404R

    Plasmid

    #200717

    Purpose

    The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    FBW7 K404 is mutated to Alanine.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304-BCL-XL-Y195F

    Plasmid

    #203573

    Purpose

    Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

    Depositor

    Abhishek Chakraborty

    Article

    Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    V5-tagged

    Expression

    Mammalian

    Mutation

    Changed Tyrosine 195 to Phenylalanine for partial…

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI_Hu_ECT2(WT)

    Plasmid

    #136330

    Purpose

    Lentiviral expression of human ECT2 WT

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    ECT2 (ECT2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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