We narrowed to 13,804 results for: TIM

1. HaloTag-WDR5

   Plasmid

   #200880

   Purpose

   Mammalian expression of human WDR5 with N-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   HaloTag-WDR5 (WDR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB80-LAMP1-mCherry-iLID

   Plasmid

   #174625

   Purpose

   Optogenetic coupling to lysosomes (LAMP1) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)
   

   Use

   Tags

   mCherry-iLID

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del

   Promoter

   Chicken beta-actin

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-MCU-D261A

   Plasmid

   #31729

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed D261 to Alanine

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-MCU-E264A

   Plasmid

   #31730

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed E264 to Alanine

   Promoter

   None

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJR106

   Plasmid

   #187244

   Purpose

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-MCU-E257A

   Plasmid

   #31727

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   Changed E257 to Alanine

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001146145)

   Plasmid

   #80198

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRY2olig-mCh-CLC

   Plasmid

   #60035

   Purpose

   Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M354I, E490G CRY2, A9D*

   Promoter

   CMV

   Available since

   Oct. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p59sheLL

   Plasmid

   #40628

   Depositor

   Simon Beddows

   Article

   Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

   Inserts

   HPV59 L1
   HPV59 L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression

   Promoter

   CMV and Polio IRES

   Available since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. p420-3

    Plasmid

    #71257

    Purpose

    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

    Insert

    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ABE7.8

    Plasmid

    #102917

    Purpose

    expresses ABE7.8 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.8
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p39sheLL

    Plasmid

    #40627

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV39 L1
    HPV39 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-Puro_siKO-2TO

    Plasmid

    #86697

    Purpose

    Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

    Depositor

    Ludovic Vallier

    Article

    Bertero et al Development. 2016 Dec 1;143(23):4405-441

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 2TO

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    U6 upstream enhancer sequence

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. Designed VEGF-binding scFv G6des13

    Plasmid

    #110213

    Purpose

    Yeast Surface Display of G6des13

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6des13
    

    Use

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH

    Plasmid

    #74289

    Purpose

    pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    H2B-GFP and oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    Ef1a

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid

    #111254

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA(sp)-mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-Syn-TRPV1-P2A-DsRed

    Plasmid

    #200830

    Purpose

    Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-DsRed (Trpv1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174640

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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