We narrowed to 7,454 results for: ski

1. pRSFDuet1-His-TEV-OTC_d1_Ub

   Plasmid

   #170729

   Purpose

   Scaffold design for studying ubiquitin interacting proteins by cryo-EM.

   Depositor

   Shang-Te Danny Hsu

   Article

   Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

   Insert

   E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   The C-terminus of OTC was truncated by removing o…

   Promoter

   T7

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIN40C.TRE.dTomato.miR-ctrl.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169316

   Purpose

   Doxycycline-inducible expression of miR-ctrl with dTomato as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN40C.SFFV.mTagBFP2.miR-ctrl

   Plasmid

   #169312

   Purpose

   Constitutive overexpression of miR-ctrl with mTagBFP2 as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   miR-ctrl
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-EGFP-TPD53

   Plasmid

   #172461

   Purpose

   Expression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFP

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD53 (TPD52L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-EGFP-TPD54

   Plasmid

   #172459

   Purpose

   Expression of untagged Tumor Protein D52 like 2 (TPD54/TPD52L2) and GFP

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00008

   Plasmid

   #166724

   Purpose

   sgRNA cloning backbone

   Depositor

   Raj Chari

   Article

   Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer10-UBC9-sh3

   Plasmid

   #168987

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-HypocratesCS

   Plasmid

   #161957

   Purpose

   Bacterial expression of inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   HypocratesCS
   

   Use

   Tags

   6xHIs tag

   Expression

   Bacterial

   Mutation

   changed cysteine 355 to serine

   Promoter

   T5

   Available since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b-His-TEV-SNAP-tag_dead_mt

   Plasmid

   #167270

   Purpose

   Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   C145A

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9FnCpf1GG

    Plasmid

    #131013

    Purpose

    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJET1.2_Nkx3-2_Anti-Sense

    Plasmid

    #124438

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJET1.2_Nkx3-2_Sense

    Plasmid

    #124437

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJET1.2_Rab28_Sense

    Plasmid

    #124441

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJET1.2_Rab28_Anti-Sense

    Plasmid

    #124442

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5 Flag RagC (D181N)

    Plasmid

    #112752

    Purpose

    express Flag-RagC (D181N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (D181N) (RRAGC Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    181N

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-sgRNA_Ago1_4

    Plasmid

    #73536

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pPN018

    Plasmid

    #91664

    Purpose

    Express sgRNA targeting human SCN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SCN2A (SCN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPN282

    Plasmid

    #91665

    Purpose

    Express sgRNA targeting human SDCCAG8

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SDCCAG8 (SDCCAG8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN283

    Plasmid

    #91666

    Purpose

    Express sgRNA targeting human SDCCAG8

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SDCCAG8 (SDCCAG8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge