-
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTK4_002
Plasmid#123811PurposeEncodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK systemDepositorInsertSV40 pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-GPI
Plasmid#65295Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP205-AAV-TRE3G-GFP-pA
Plasmid#164789PurposeAAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT86
Plasmid#108965PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tagDepositorTypeEmpty backboneUseTagsOmpA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-SOS
Plasmid#208275PurposeExpresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTM-BPL
Plasmid#173766PurposeFluorescent labeling of the nuclear envelopeDepositorInsertPDGF receptor TM domain, Biotin protein ligase
UseTagsFLAG and Signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mito-Lapronic/pGW1
Plasmid#140754PurposeFRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate RatioDepositorInsertMito-Lapronic
UseTagsMitochondrial Sequence SignalExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FKBP_LD 0_TEV
Plasmid#58877PurposeMESA protease chain with Rapamycin FKBP ectodomain and no linker domainDepositorInsertMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
UseAAVTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD
Plasmid#170124PurposeLentiviral vector carrying the MCP-ADAR2 with a nuclear export signalDepositorInsertMCP-ADAR2-DD-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-PhoCl-mCherry
Plasmid#87691PurposeExpress NLS-PhoCl-mCherry in mammalian cellsDepositorInsertNLS-PhoCl-mCherry
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
CyTERM-mGFP
Plasmid#62237PurposeER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.DepositorInsertCyTERM-mGFP
UseTagsmGFPExpressionMammalianMutationsignal anchor of rabbit cytochrome p450 and monom…PromoterCMVAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVH1002-Tier2(pUC ori)
Plasmid#169600PurposeTier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM
Plasmid#115554PurposeRed fluorescent voltage indicator for neuronal expressionDepositorInsertVARNAM
UseAAVTagsGolgi and ER export signalExpressionMutationPromoterhuman SynapsinAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-GPI
Plasmid#65299Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectMutationPromoterPolyhedrin (LRP6)/p10 (MESD)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b- mKate2-KDEL-pA
Plasmid#109494PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequenceDepositorInsertactc1b- mKate2-KDEL-pA
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-C1C2-TS-EYFP
Plasmid#35520DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsTrafficking signal and EYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1m_v1
Plasmid#97083PurposeEncodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoformDepositorInsertsgRNA NEAT1m_v1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only