We narrowed to 5,107 results for: PEP

1. 10279-M28-663

   Plasmid

   #225662

   Purpose

   Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-A24

   Plasmid

   #135507

   Purpose

   Mammalian expression of myc-tagged HLA-A*024:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-UTX-F-MT2

   Plasmid

   #40619

   Purpose

   Expression of enzyme-dead UTX in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTX (KDM6A Human)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   changed Histidine 1146 and Glutamic Acid 1148 to …

   Promoter

   CMV

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. β₂AR-mCFP

   Plasmid

   #38260

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   β₂AR (ADRB2 Human)
   

   Use

   Tags

   Flag, mCFP, and signal peptide sequence (MKTIIALS…

   Expression

   Mammalian

   Mutation

   diglycine linker (replacing terminator codon)

   Promoter

   CMV

   Available since

   Aug. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

   Plasmid

   #232122

   Purpose

   A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   Tcf4ΔN (Tcf4 Mouse)
   

   Use

   Gateway cloning

   Tags

   myc

   Expression

   Mutation

   lacks aa 1-56

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR25-DuET

   Plasmid

   #213277

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR25 (GPR25 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. STR-KDEL_PGRN-SBP-mCherry

   Plasmid

   #180439

   Purpose

   Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

   Depositor

   Shawn Ferguson

   Article

   Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

   Insert

   Progranulin (GRN Human)
   

   Use

   Tags

   SBP-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-myc-EphB6

    Plasmid

    #200984

    Purpose

    Mammalian expression plasmid for myc-tagged EphB6

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB6 (EPHB6 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-HemmarR

    Plasmid

    #31222

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 May 23. (

    Insert

    CD4-tdTom (CD4 Aequorea victoria, Fly, Human)
    

    Use

    Tags

    CD4 and tdTomato

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid

    #113955

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, one base pair dele…

    Promoter

    cmv

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-BG505.SOSIP-gp160-754*

    Plasmid

    #123274

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 3xFlag IFIT2

    Plasmid

    #53555

    Purpose

    mammalian expression of IFIT2

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT2 (IFIT2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET20b_Pa-PiuA

    Plasmid

    #128955

    Purpose

    expresses Pseudomonas aeruginosa PiuA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEX-SP-YFP-STIM1(23-685)

    Plasmid

    #18857

    Depositor

    Tobias Meyer

    Article

    Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    YFP is inserted between residues 1-23 (signal pep…

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEarleyGate 100RMOA mPing

    Plasmid

    #196137

    Purpose

    Measures mPing transposition in plants

    Depositor

    Charles Nathan Hancock

    Article

    Renken et al MicroPubl Biol. 2023 May 16;2023:10.1791

    Inserts

    Pong TPase LA T2A ORF1SC1 ONE
    mPing
    

    Use

    Binary

    Tags

    Expression

    Plant

    Mutation

    TPase NES sequence mutated L418A, L420A; T2A pept…

    Promoter

    35S and RPS5a

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT2-4xUAS:P2A-EGFP

    Plasmid

    #127591

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with EGFP through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ADORA3-DuET

    Plasmid

    #213176

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA3 (ADORA3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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