We narrowed to 11,766 results for: nsf

1. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

   Plasmid

   #71217

   Purpose

   dCas9 fused to human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B catalytic domain
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAd-Track HA PGC-1 alpha

   Plasmid

   #14427

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Adenoviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Last four amino acids of PGC-1a removed to make t…

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

   Plasmid

   #123194

   Purpose

   binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
   

   Use

   Luciferase

   Expression

   Plant

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Raichu-OsRac1 CA

   Plasmid

   #133264

   Purpose

   Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(G19V) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 G19V

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Raichu-OsRac1 DN

   Plasmid

   #133263

   Purpose

   Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

   Depositor

   Yoji Kawano

   Article

   Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

   Insert

   OsRac1(T24N) (LOC4325879 Oryza sativa)
   

   Tags

   CFP-lipid and Venus-CRIB

   Expression

   Plant

   Mutation

   OsRac1 T24N

   Promoter

   Ubi

   Available Since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-XS-VWF

   Plasmid

   #64847

   Purpose

   Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

   Depositor

   Sriram Neelamegham

   Article

   Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

   Insert

   Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Synthetic, Human)
   

   Tags

   7X His, Cerulean, and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh55

   Plasmid

   #106728

   Purpose

   expression of gRNA targeting B4GALT7

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B4GALT7 (B4GALT7 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pIE-hα2M-H6

    Plasmid

    #128492

    Purpose

    Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBullet-tgn-c

    Plasmid

    #53072

    Purpose

    destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. gh139

    Plasmid

    #106809

    Purpose

    expression of gRNA targeting EXTL3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    EXTL3 (EXTL3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5-Flag-EPLIN alpha delta LIM

    Plasmid

    #40933

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN alpha (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted- amino acids 221-295

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pBullet-end-c

    Plasmid

    #53074

    Purpose

    destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. mitoTALECD for_atp1_1397CN

    Plasmid

    #186203

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SopB-EGFP

    Plasmid

    #183655

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CreLite

    Plasmid

    #131785

    Purpose

    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-ms-c-Myc-V394D

    Plasmid

    #50775

    Purpose

    retroviral expression of mouse c-Myc V394D mutant

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc V394D (Myc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V394D (does not bind to Miz-1)

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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