We narrowed to 10,449 results for: T7

1. pET-DEST42-ARL1-V5-His6

   Plasmid

   #67416

   Purpose

   Expression of hArl1 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 231 C to T; silent mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET-DEST42-ARL5A-V5-His6

   Plasmid

   #67420

   Purpose

   Expression of hArl5 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL5A (ARL5A Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 70 C to T; silent mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET-DEST42-ARL14-V5-His6

   Plasmid

   #67427

   Purpose

   Expression of hArl14 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL14 (ARL14 Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 172 C to T; aa L58F

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET-DEST42-ARL11-V5-His6

   Plasmid

   #67428

   Purpose

   Expression of hArl11 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL11 (ARL11 Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 442 T to C; aa C148R

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-DEST42-ARFRP1-V5-His6

   Plasmid

   #67429

   Purpose

   Expression of hArfrp with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARFRP1 (ARFRP1 Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. 213-petDuet1-GBD-LUMP--cdc42

   Plasmid

   #65973

   Purpose

   Dual expression of GBD-LUMP and cdc42

   Depositor

   Gerard Marriott

   Article

   Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

   Inserts

   LUMP
   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   GBD (GTPase binding domain from ACK1; residues 44…

   Expression

   Bacterial

   Mutation

   constitutively active Cdc42 (Q61L)

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-22HT-MSI1(7-192)Q30D

   Plasmid

   #60346

   Purpose

   Q30D mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   Q30D; contains amino acids 7-192 of Msi1

   Promoter

   T7

   Available since

   Dec. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET-22HT-MSI1(7-192)R53E

   Plasmid

   #60352

   Purpose

   R53E mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   R53E; contains amino acids 7-192 of Msi1

   Promoter

   T7

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET-22HT-MSI1(7-192)G64A

   Plasmid

   #60351

   Purpose

   G64A mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   G64A; contains amino acids 7-192 of Msi1

   Promoter

   T7

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pET-22HT-MSI1(7-192)G35D

    Plasmid

    #60347

    Purpose

    G35D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET-22HT-MSI1(7-192)G35E

    Plasmid

    #60348

    Purpose

    G35E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET-22HT-MSI1(7-192)H83F

    Plasmid

    #60349

    Purpose

    H83F mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83F; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcdna3 T7Jip1(282-470)

    Plasmid

    #51703

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 1-281 and 471-660

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcdna3 T7Jip1(1-126)

    Plasmid

    #51702

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 127-660

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

16. hSRAP.HMG6.wt.Dlnk.a13-236

    Plasmid

    #49814

    Purpose

    expression vector for MBP-SRAP(13-236) fusion

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein (SRA1 Human)
    

    Use

    Tags

    E. coli Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTYB-pLAC-F112S

    Plasmid

    #48727

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Phenylalanine 12 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pTYB-pLAC-L108S

    Plasmid

    #48725

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Leucine 108 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pTYB1-pLAC-I98S

    Plasmid

    #48723

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 98 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTYB1-pLAC-I73S

    Plasmid

    #48722

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 73 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pTYB1-pLAC-V69S

    Plasmid

    #48721

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Valine 69 to Serine

    Promoter

    T7

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

22. BRCA1-(219-408)

    Plasmid

    #35067

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-408

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

23. IFI16-(192-393)-HinA-E222A-E272A-E381A

    Plasmid

    #35061

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E222A, E272A, a…

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

24. 2Bc-T MBP_BoMoC(ed)_6xH

    Plasmid

    #186461

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Upton et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GLP1R-DuET

    Plasmid

    #213253

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GLP1R (GLP1R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGP-CMV-GCaMP6f

    Plasmid

    #40755

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6f
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Use

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge