We narrowed to 34,848 results for: CaS;

1. pBLO43.3.-6_human ProCas9Flavi-6

   Plasmid

   #121630

   Purpose

   U6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3s-Cas9-HF1

   Plasmid

   #104173

   Purpose

   Cas9-HF1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   Cas9-HF1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCaSpeR-act5cB-LexAQF

   Plasmid

   #61310

   Purpose

   Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA_DBD::QF2_AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

   Plasmid

   #192350

   Purpose

   L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   AATG_dttLbCas12a-i_TTCG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R; E925Q

   Promoter

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RCAS (A) Six1 (CT#664)

   Plasmid

   #13876

   Depositor

   Cliff Tabin

   Article

   Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

   Insert

   Six1 (Six1 Mouse)
   

   Use

   Retroviral; Avian expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. p3s-eCas9-1.1

   Plasmid

   #104172

   Purpose

   eCas9-1.1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   eCas9-1.1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-CCHD (p130Cas)

   Plasmid

   #145133

   Purpose

   Expresses CCH domain from p130Cas with an mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   p130Cas CCH domain (BCAR1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti Cas9/SB100

    Plasmid

    #155294

    Purpose

    Lentiviral expression of Cas9 and SB100

    Depositor

    Martin Carroll

    Insert

    SB100X-P2A-spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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