We narrowed to 7,568 results for: Lif

1. pPUR-mU6-sgRNA-Sirius-8XPP7

   Plasmid

   #121943

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   sgRNA-Sirius-8XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

   Plasmid

   #60909

   Purpose

   A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   K560-E236A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPLP1H-mCf

   Plasmid

   #229984

   Purpose

   LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPLP2H-mCf

   Plasmid

   #229985

   Purpose

   LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPLP3H-mCf

   Plasmid

   #229987

   Purpose

   LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-EF1a_NKX2-1

   Plasmid

   #221484

   Purpose

   NKX2-1 short isoform lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ttAtCas12a+int

   Plasmid

   #182384

   Purpose

   Gives very high editing efficiency in barley. Also works in Brassica oleracea

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   LbCas12a coding sequence with D156R and Arabidopsis introns
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R + 8 Arabidopsis introns added

   Promoter

   None

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag FoxO3

    Plasmid

    #14937

    Depositor

    Joan Massague

    Article

    Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

    Insert

    FoxO3 (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p159 pPr-luc

    Plasmid

    #8392

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    probasin promoter (Pbsn Rat)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pCMU-ACTLr

    Plasmid

    #61193

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO_NES_HaloTag9

    Plasmid

    #175537

    Purpose

    Strict cytosolic expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD/HisB (TIREVOL)-sfGFP

    Plasmid

    #189675

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-hCDC6p-Venus

    Plasmid

    #212665

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-E p27 (CC#631)

    Plasmid

    #15192

    Depositor

    Connie Cepko

    Article

    Dyer et al J Neurosci. 2001 Jun 15. 21(12):4259-71.

    Insert

    p27 (Cdkn1b Mouse)
    

    Use

    Retroviral

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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