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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Promoter

U6

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-7

Plasmid

#126055

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b S179AS180A

Plasmid

#118327

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Publication

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rap1b S179AS180A (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

S179AS180A

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22 / PP1gamma

Plasmid

#113511

Purpose

Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Inserts

SDS22 (PPP1R7 Human)
PP1gamma (PPP1CC Human)

Tags

6*His

Expression

Insect

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002 DA-MBP

Plasmid

#102832

Purpose

Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002 DA-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Reactive aspartic acid has been mutated to an ala…

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMDC43-FIT2

Plasmid

#96993

Purpose

Express GFP-tagged mouse FIT2 gene in plants.

Depositor

Kent Chapman

Publication

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Tags

GFP

Expression

Plant

Mutation

E2D (please see depositor comments below)

Promoter

35S

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(T308A/S473A)

Plasmid

#86578

Purpose

Expresses human full-length Akt1(T308A/S473A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

T308A/S473A phosphomutant

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Publication

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4a-ISH-2kb

Plasmid

#68029

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4A (Sema4a Mouse)

Use

Mrna in situ hybridization probe

Mutation

a 2kb cDNA fragment for ISH probe synthesis

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+17

Plasmid

#61096

Purpose

Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

Depositor

Robin Shaw

Publication

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 17, excluding exon 7, 11, 13-16

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Publication

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-1282-1792

Plasmid

#40960

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1281

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
TAL2386

Plasmid

#36795

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Left (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2358

Plasmid

#36771

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX3-TALEN_Left (CBX3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2359

Plasmid

#36772

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX3-TALEN_Right (CBX3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
XE46 dnWnt-1

Plasmid

#16860

Depositor

Randall Moon

Publication

Hoppler et al Genes Dev. 1996 Nov 1. 10(21):2805-17.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dnWnt-1 (Wnt1 Mouse)

Use

Xenopus expression

Mutation

truncated gene

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
FRB-GFP-GCN4-ppKin14

Plasmid

#240427

Purpose

-end oriented kinesin for rapalog induced pulling experiments.

Depositor

Anna Akhmanova

Publication

Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ppKin14Vib

Tags

FRB, GCN4, and GFP

Expression

Mammalian

Mutation

deleted amino acids 1-850

Available since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNA 3C myc scfvFLAG

Plasmid

#234990

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Publication

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvFLAG

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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