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hp-66%GC-40bp

Plasmid

#169200

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-20bp

Plasmid

#169201

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-75%GC-40bp

Plasmid

#169202

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

75%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-100%GC-20bp

Plasmid

#169203

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

100%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Left-half-120bp

Plasmid

#169204

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Left-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Right-half-120bp

Plasmid

#169205

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Right-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Left-half-120bp

Plasmid

#169206

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Left-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Right-half-120bp

Plasmid

#169207

Purpose

50-0-50%GC-Right-half-120bp-hairpin

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Right-half-120bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-20bp

Plasmid

#169196

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp

Plasmid

#169197

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-40bp-Revbindsite

Plasmid

#169198

Purpose

Force-activating hairpin for 5'-to-3' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-40bp-hairpin-binding-site-reversed

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-66%GC-20bp

Plasmid

#169199

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

66%GC-20bp-hairpin

Use

Unspecified

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-no charge

Plasmid

#160532

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, E337A, N338A, K341A, N342A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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