We narrowed to 14,168 results for: eng

Showing: 4321 - 4340 of 14168 results

Results per page:

Sort results by:

pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

Plasmid

#205549

Purpose

Express EPN24-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKII-STChRger2-TS-EYFP

Plasmid

#129393

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Publication

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Publication

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pTXG-hTDG

Plasmid

#52281

Purpose

Bacterial expression of human TDG C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDUET-ctCPR-BovAMO

Plasmid

#20113

Depositor

Jay Keasling

Publication

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase, and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDUET-ctCPR-A17AMO

Plasmid

#20115

Depositor

Jay Keasling

Publication

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCYB-TnsA

Plasmid

#17356

Depositor

Nancy Craig

Publication

Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

Insert

TnsA (tnsA E. coli transposon Tn7)

Tags

Intein-Chitin binding domain

Expression

Bacterial

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pHiUGE GS-gRNA (hSyn-Cre) mTubb3

Plasmid

#200369

Purpose

Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Mouse Tubb3 gRNA and Cre

Expression

Mammalian

Mutation

NA

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-SA-neo-CAGGS-PE2-2A-GFP

Plasmid

#180014

Purpose

Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

Depositor

Dirk Hockemeyer

Publication

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2-2A-GFP

Use

CRISPR; Human genome engineering

Tags

GFP

Promoter

CAG

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBx1-VHH template3-3XMyc-Spacer

Plasmid

#176210

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Publication

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template3-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101101

Purpose

Encodes dCas9(N) fused to transmembrane tether

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
978 pSG5L HA PTEN 1-373

Plasmid

#10766

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-373.

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
lentiCRISPRv2-sgCTL

Plasmid

#234771

Purpose

Non Targeting Control

Depositor

Judy Lieberman

Publication

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Non Targeting Control sgRNA

Use

Lentiviral

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His-CL7-2NLS-SpyCas9(dS)-A22p3star

Plasmid

#222970

Purpose

Bacterial expression of A22p-fused SpyCas9 construct

Depositor

Jennifer Doudna

Publication

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His-CL7-2NLS-SpyCas9(dS)-A22p3

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Publication

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge