We narrowed to 7,129 results for: trac

1. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-CA-rtTA Adv

   Plasmid

   #20910

   Purpose

   piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   rtTA-ON-Adv
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAP05

   Plasmid

   #102920

   Purpose

   Direct cloning and broad-host-range heterologous expression vector

   Depositor

   Bradley Moore

   Article

   Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSKI

   Plasmid

   #232484

   Purpose

   pSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

   Depositor

   Carlos Silva García

   Article

   Dinneen et al bioRxiv [Preprint]. 2024 Dec 10:2024.12.

   Insert

   pSKI
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TetO-FUW-pgk-puro

   Plasmid

   #85747

   Purpose

   tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR CD22-Megf10 CAR

   Plasmid

   #113016

   Purpose

   for production of lentivirus encoding CD22 CAR-P Megf10

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD22 CAR-P Megf10 intracellular domain
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ134B2.0

   Plasmid

   #167158

   Purpose

   Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CFP-tTA-ER-C3

   Plasmid

   #134569

   Purpose

   Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSK-Sasg

   Plasmid

   #214350

   Purpose

   Expresses cloning backbone for SlugCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMP71-CD40L

    Plasmid

    #174595

    Purpose

    Stably expresses the extracellular and transmembrane domain of murine CD40L

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    CD40L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAM5057

    Plasmid

    #120085

    Purpose

    Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

    Insert

    RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZac2.1 GfaABC1D NAPA-A SV40

    Plasmid

    #92281

    Purpose

    FRET probe; extracellular membrane tethered eGFP

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Astrocyte
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

    Plasmid

    #45565

    Purpose

    Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    I-Sce I Nuclease
    BFP
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1 hSynapsin1 NAPA-N SV40

    Plasmid

    #92282

    Purpose

    FRET probe; extracellular synapse targeted mCherry

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCLE NICD3

    Plasmid

    #26894

    Depositor

    Nicholas Gaiano

    Article

    Dang et al Dev Neurosci. 2006;28(1-2):58-69.

    Insert

    NICD3 (NOTCH3 Human)
    

    Use

    Retroviral

    Tags

    Alkaline Phosphatase and IRES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-mCh-kCAAX

    Plasmid

    #112267

    Purpose

    ITRACK Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    mCherry with KRas membrane targeting domain
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ135B2.0

    Plasmid

    #167159

    Purpose

    Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ136B2.0

    Plasmid

    #167160

    Purpose

    Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFG036 + pFG051

    Plasmid

    #137997

    Purpose

    Transposon mutagenesis plasmid and the cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    Tn5 tnp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge