We narrowed to 6,963 results for: RAP

1. pMOD3-mClover-Sh_ble

   Plasmid

   #120329

   Purpose

   Bacterial mClover promoter trap

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   mClover
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D103N (see comment below)

   Promoter

   (None)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4xCSL-luciferase

   Plasmid

   #41726

   Depositor

   Raphael Kopan

   Article

   Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

   Insert

   4xCSL binding sites
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains a multimerized high affinity CSL binding…

   Promoter

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEZT-BM

   Plasmid

   #74099

   Purpose

   Multi-purpose rapid titer bacmam expression vector

   Depositor

   Ryan Hibbs

   Article

   Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Bacmam, baculovirus

   Tags

   EGFP following p10 promoter

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, p10

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMito-mCherry-FRB

   Plasmid

   #59352

   Purpose

   Expresses mCherry-tagged MitoTrap in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

   Type

   Empty backbone

   Use

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. iRFP-FRB-Rab5

   Plasmid

   #51612

   Purpose

   Recruiter to Rab5-positive endosomes

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   FRB
   Rab5
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. iRFP-FRB-Rab7

   Plasmid

   #51613

   Purpose

   Recruiter to Rab7-positive endosomes

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Inserts

   FRB
   Rab7
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-X1-Blast-rLAMP1-eGFP

   Plasmid

   #109028

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   rLAMP1
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EIF-1a promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-TTP(C147R)-GG-PUF

   Plasmid

   #52138

   Purpose

   Receiving vector for PUM-HD assembly and expression in mammalian cells

   Depositor

   Huimin Zhao

   Article

   Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

   Type

   Empty backbone

   Use

   Tags

   (GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2KXIGdeltaIn-MCS-birA-tagRFP

   Plasmid

   #58378

   Purpose

   tol2 transgenesis for biotin based TRAP - BirA ligase

   Depositor

   Didier Stainier

   Article

   Housley et al Development. 2014 Oct;141(20):3988-93. d

   Insert

   birA tagRFP
   

   Use

   Tags

   tagRFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2KXIGdeltaIn-MCS-birA-EGFP

    Plasmid

    #58377

    Purpose

    tol2 transgenesis for biotin based TRAP - BirA ligase

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    birA EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Reporter

    Plasmid

    #36008

    Depositor

    Larry Gerace

    Article

    Ohba et al J Cell Biol. 2004 Dec 20;167(6):1051-62.

    Insert

    LAP2β-TM
    

    Use

    Reporter

    Tags

    11kD FRB and EGFP

    Expression

    Mammalian

    Mutation

    FRB is residues 2025-2114 of FRAP, LAP2β-TM is re…

    Promoter

    CMV

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pGBT-R15

    Plasmid

    #31826

    Depositor

    Stephen Ekker

    Article

    Petzold et al Proc Natl Acad Sci U S A. 2009 Nov 3;106

    Insert

    Tol2 (t2gtlp4 Zebrafish)
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDifferentiation

    Plasmid

    #228393

    Purpose

    intended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

    Insert

    pLambda-luxR+LVAtag__pLux-mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Epac-S-H127

    Plasmid

    #170343

    Purpose

    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Insert

    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Epac-S-H151

    Plasmid

    #170346

    Purpose

    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Insert

    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    SP6

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEL1584

    Plasmid

    #139875

    Purpose

    Expression plasmid of rings 2 and 3 of LetB for crystallography

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(159-383)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Promoter

    Available since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-X1-Neo-GFP-FAM134B

    Plasmid

    #109026

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-X1-hygro-mCherry-RAMP4

    Plasmid

    #118391

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFLAG-SURF4

    Plasmid

    #170680

    Purpose

    Expresses FLAG-tagged SURF4 in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    SURF4
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Lys-eMagA-EGFP

    Plasmid

    #162246

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    p18-eMagA-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-BE2

    Plasmid

    #73020

    Purpose

    Expresses BE2 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Use

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. HA-BICD2(1-500)-FRB

    Plasmid

    #174648

    Purpose

    Rapalog-inducible coupling to dynein adaptor BICD2

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    HA-BICD2(1-500)-FRB
    

    Use

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    mmBICD2(aa1-500): Lys213Glu

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS2 Notch1 ICv-6MT

    Plasmid

    #41730

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Intracellular Domain (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 protein beginning at Vali…

    Promoter

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p667-UBC-SCYL1-V5-miniTurbo_IDG-K

    Plasmid

    #189911

    Purpose

    Gateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    SCYL1 (SCYL1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMito-GBPen

    Plasmid

    #128269

    Purpose

    Expression of mitochondrially targeted GBPen (GFP Nanobody)

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Insert

    Mito-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge