We narrowed to 23,099 results for: crispr

1. pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

   Plasmid

   #244834

   Purpose

   Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-36XMYC array

   Plasmid

   #236380

   Purpose

   Expression of array of 36 crRNAs targeting MYC

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   36xMYC crRNA array (MYC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-48xEFNA1 array

   Plasmid

   #236382

   Purpose

   Expression of array of 48 crRNAs targeting EFNA1

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   48xEFNA1 crRNA array (EFNA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-48xGPBP1 array

   Plasmid

   #236383

   Purpose

   Expression of array of 48 crRNAs targeting GPBP1

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   48xGPBP1 crRNA array (GPBP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-48xHSPA1A array

   Plasmid

   #236377

   Purpose

   Expression of array of 48 crRNAs targeting HSPA1A

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   48xHSPA1A crRNA array (HSPA1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRGEB32-CrU6-2-35S

   Plasmid

   #226706

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from CaMV 35S promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrU6-2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-N-Solo-mStayGold-LMNB1

   Plasmid

   #227328

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-C-Solo-mScarlet-MAPRE1

   Plasmid

   #227323

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-KO-Puro-TP53

   Plasmid

   #227319

   Purpose

   Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-C-Solo-mStayGold-EZR

    Plasmid

    #227294

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    EZR Homology Arms flanking a mStayGold Tag (EZR Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid

    #227278

    Purpose

    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRGEB32-CrU6-2-UBI

    Plasmid

    #226707

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrU6-2 promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrU6-2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-Solo-mStayGold-H3C2

    Plasmid

    #227334

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid

    #227335

    Purpose

    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid

    #227333

    Purpose

    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-N-Solo-mStayGold-MAP4

    Plasmid

    #227296

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-C-Solo-mStayGold-CEP192

    Plasmid

    #227289

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-C-Solo-mStayGold-ARL13B

    Plasmid

    #227277

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-mStayGold-EFS-Puro-CEP192

    Plasmid

    #227290

    Purpose

    Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AA020

    Plasmid

    #216004

    Purpose

    Fragmid fragment: (Cas protein) binds PP7 stem loops

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    PP7 Core Protein (PCP)_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AA390

    Plasmid

    #215957

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control reporter

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AA246

    Plasmid

    #215946

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD55_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AA233

    Plasmid

    #215941

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD274_v1 [Sp]; trRNA_v14 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AA033

    Plasmid

    #216013

    Purpose

    Fragmid fragment: (Cas protein) deactivated Cas

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dCas9-NG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AA244

    Plasmid

    #215945

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AA363

    Plasmid

    #215955

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dU6:3_v1; BsmBI_v14; BsmBI_v1; trRNA_v1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AA303

    Plasmid

    #216071

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AA035

    Plasmid

    #216015

    Purpose

    Fragmid fragment: (Cas protein) deactivated Cas

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dCas9-SpRY_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AA034

    Plasmid

    #216014

    Purpose

    Fragmid fragment: (Cas protein) deactivated Cas

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dCas9-SpG_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AA242

    Plasmid

    #215943

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v1 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AA243

    Plasmid

    #215944

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v5 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AA310

    Plasmid

    #215950

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control lethal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AA232

    Plasmid

    #215940

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface with 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v4 [Sp]; trRNA_v14 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AA238

    Plasmid

    #215942

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD47_v4 [Sp]; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AA054

    Plasmid

    #215932

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AA285

    Plasmid

    #215947

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AA021

    Plasmid

    #215929

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AA023

    Plasmid

    #215931

    Purpose

    Fragmid fragment: (guide cassette) guide expression; positive control cell surface

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AA022

    Plasmid

    #215930

    Purpose

    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; sgCD4_v3 [Sp]; trRNA_v14 [Sp]; CS2
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. AA016

    Plasmid

    #215927

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AA017

    Plasmid

    #215928

    Purpose

    Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v14 [Sp]; CS2
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AA102

    Plasmid

    #215935

    Purpose

    Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AA403

    Plasmid

    #215960

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [Cas7-11]; BsmBI_v16; BsmBI_v12
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AA107

    Plasmid

    #215936

    Purpose

    Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMV_AA091

    Plasmid

    #216106

    Purpose

    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    gRNA expression with modified trRNA to recruit activators via PP7 tags
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AA048

    Plasmid

    #216016

    Purpose

    Fragmid fragment: (Cas protein) deactivated Cas

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dCas12a (D908A)_v1.1 [EnAs]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRDB_131

    Plasmid

    #216095

    Purpose

    Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD97, CD4, CD26, CD274 guides
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

    Plasmid

    #214882

    Purpose

    Lentiviral vector encoding RfxCas13d targeting PROX guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crPROX-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pDonor-PRF1-Exon3

    Plasmid

    #209075

    Purpose

    Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    PRF1-Exon3 HDRT (PRF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge