We narrowed to 31,002 results for: LIS;

1. pYPQAT08

   Plasmid

   #223380

   Purpose

   T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpRY-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-KG-WASP-GBD

   Plasmid

   #30113

   Depositor

   David Sacks

   Article

   Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

   Insert

   WASP-GBD (WAS, WAS Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains the Cdc42-interacting domain of WASP (aa…

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. P2A-NLS-tdtomato-EPG backbone

   Plasmid

   #215548

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-DeltaJunD

   Plasmid

   #68547

   Purpose

   AAV expression of DeltaJunD

   Depositor

   Eric Nestler

   Article

   Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

   Insert

   DeltaJunD-IRES-GFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   truncated splice variant

   Promoter

   CMV

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

6. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. DL015:CMV::iPAK4

   Plasmid

   #177880

   Purpose

   ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-cytoFLARE1.0-TEV

   Plasmid

   #234517

   Purpose

   Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

   Depositor

   Wenjing Wang

   Article

   Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

   Insert

   EGFP-P2A-CaM-V5-TEVp
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

   Plasmid

   #171436

   Purpose

   Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-Lifeact
   

   Expression

   Mammalian

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DL016:CMV::eGFP-iPAK4

    Plasmid

    #177881

    Purpose

    eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Promoter

    chicken actin

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

12. optoAKT

    Plasmid

    #207164

    Purpose

    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

    Depositor

    Kai Zhang

    Article

    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

    Insert

    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FnR8-10

    Plasmid

    #182569

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. psgRNAv1-empty

    Plasmid

    #171611

    Purpose

    sgRNA_v1 plasmid for AsCas12f1 in bacteria

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1_sgRNA_1
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-anti-mCherry(LaM6) PAGER(FL)

    Plasmid

    #230023

    Purpose

    Expresses anti-mCherry PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-LaM6-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230018

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gi) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM4D
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-SFFV-anti-mCherry(LaM6) PAGER(Gi)

    Plasmid

    #230021

    Purpose

    Lentiviral vector expressing anti-mCherry PAGER(Gi) in human T cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM4D
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT07

    Plasmid

    #223379

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCBFRE-luc

    Plasmid

    #26897

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

20. Cyto_K20

    Plasmid

    #133862

    Purpose

    Expression of cytoplasmic Ribosome stalling reporter

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Cyto_K20
    

    Tags

    EGFP and RFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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