We narrowed to 5,111 results for: codon optimized

1. CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171381

   Purpose

   Expression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13Y.1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC22A5

   Plasmid

   #132048

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A5 (SLC22A5 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDONR221_SLC39A4

   Plasmid

   #132069

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC39A4 (SLC39A4 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCBh_3x Flag-NLS-enOsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197026

   Purpose

   Vector encoding a human codon-optimized enOsCas12f1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized enOsCas12f1
   

   Expression

   Mammalian

   Mutation

   D52R / T132R

   Promoter

   CBh, CMV, hU6

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC25A12

   Plasmid

   #131938

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A12 (SLC25A12 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR221_SLC2A9

   Plasmid

   #131879

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A9 (SLC2A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

   Plasmid

   #193769

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1ΔR198A/R210A (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC38A3

   Plasmid

   #132142

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC38A3 (SLC38A3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDONR221_SLC7A10

    Plasmid

    #132232

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A10 (SLC7A10 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR221_SLC39A1

    Plasmid

    #132058

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC39A1 (SLC39A1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pDONR221_SLC30A7

    Plasmid

    #132148

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A7 (SLC30A7 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAB1677 REPAIR.t3

    Plasmid

    #176320

    Purpose

    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Inserts

    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    E488Q, only the deaminase domain (aa 276-702 are …

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLCO1B1

    Plasmid

    #132188

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO1B1 (SLCO1B1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDONR221_SLC4A4

    Plasmid

    #132260

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC4A4 (SLC4A4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 19, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SLC38A1

    Plasmid

    #132095

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC38A1 (SLC38A1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET28b-SpG-His

    Plasmid

    #179318

    Purpose

    Plasmid for bacterial expression and purification of SpG-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpG
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC7A1

    Plasmid

    #131925

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A1 (SLC7A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR221_SLC30A5

    Plasmid

    #132077

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A5 (SLC30A5 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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