We narrowed to 5,331 results for: pcdna3.1

1. CoV2-N-D377Y

   Plasmid

   #177957

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D377Y) (N )
   

   Expression

   Mammalian

   Mutation

   D377Y

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pspCas9-ABE-3'UTR-sgRNA-2xMS2

   Plasmid

   #132554

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
   

   Expression

   Mammalian

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJO428

   Plasmid

   #111141

   Purpose

   URT-type vector for Val-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3-HA/Bmal L95E

   Plasmid

   #47344

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E

   Promoter

   CMV

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. Myc-delta

   Plasmid

   #118037

   Purpose

   GABAA receptor expression (delta subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Article

   Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

   Insert

   GABRD (Gabrd Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-p160(1-200)

   Plasmid

   #43

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   p160 myb binding protein (Mybbp1a Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

8. hMEF2A K403R

   Plasmid

   #118355

   Purpose

   This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K403R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-p160(400-580)

   Plasmid

   #45

   Depositor

   Bruce Spiegelman

   Article

   Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

   Insert

   p160 myb binding protein (Mybbp1a Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   expresses only aa400-580

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-p160(200-400)

    Plasmid

    #44

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Expresses only aa200-400

    Available Since

    July 21, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3 RL23Cm6

    Plasmid

    #109367

    Purpose

    Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin-mGluR6 chimera (RHO, GRM6 Human)
    

    Tags

    1D4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-N-P13L

    Plasmid

    #177946

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P13L) (N )
    

    Expression

    Mammalian

    Mutation

    P13L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV2-N-P80R

    Plasmid

    #177947

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R) (N )
    

    Expression

    Mammalian

    Mutation

    P80R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CoV2-N-S235F

    Plasmid

    #177956

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (S235F) (N )
    

    Expression

    Mammalian

    Mutation

    S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FLAG-FLJ23445

    Plasmid

    #15429

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    FLJ23445 (Thoc7 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

16. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Opto-GPR173

    Plasmid

    #106058

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR173 (GPR173 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Opto-GPR31

    Plasmid

    #106014

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR31 (GPR31 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL4-DBD-TBX1wt-IDR

    Plasmid

    #145254

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1wt-IDR (TBX1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge