We narrowed to 37,893 results for: KAN

1. p005-RFP-weak

   Plasmid

   #108316

   Purpose

   Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   RFP under controlf of weak synthetic constitutive promoter pFAB4024
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pFAB4024

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCML 220

   Plasmid

   #98588

   Purpose

   pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. ReNL-Actin_pcDNA3

   Plasmid

   #89531

   Purpose

   Red color variant of bright luminescent protein, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Use

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. tubulin-OeNL_pcDNA3

   Plasmid

   #89523

   Purpose

   Orange color variant of bright luminescent protein, fused to b-tubulin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   b-tubulin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kohinoor-Fibrilarin/pcDNA3

   Plasmid

   #67781

   Purpose

   Fibrilarin-targetted Kohinoor for mammalian cell expression

   Depositor

   Takeharu Nagai

   Article

   Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

   Insert

   Kohinoor-Fibrilarin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. lenti-CMV-TFAM-BioID2-IRES-Puro

   Plasmid

   #211465

   Purpose

   Lentivirus backbone expressing human TFAM fused with BioID2

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   TFAM-BioID2
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIMcpf1

   Plasmid

   #153034

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRH-1328 LV EF1a-CD20 IRES-EGFP

   Plasmid

   #201918

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD20
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    2x35S and StUbi-3P

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCN-mBFP

    Plasmid

    #63847

    Purpose

    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

    Depositor

    Howard Salis

    Article

    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

    Insert

    mBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter and RBS was swapped. His-tag was removed…

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-mTagBFP2

    Plasmid

    #122373

    Purpose

    Expresses mTagBFP2 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sec61b-V5-TurboID

    Plasmid

    #166971

    Purpose

    Expresses ER targeted TurboID for secretome biotinylation

    Depositor

    Hyun-Woo Rhee

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

    Plasmid

    #187186

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Y90F, V91F

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. LDB-3-p65

    Plasmid

    #158984

    Purpose

    Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

    Depositor

    Liangcai Gu

    Article

    Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

    Insert

    LDB-3-p65
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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