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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,133 results for: codon optimized

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Showing: 4361 - 4380 of 5133 results

  9. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

    Plasmid
    #193769
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1ΔR198A/R210A (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193769-plasmid-map-sequence-id-380211

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  10. pCW-ND-Cdt1Δ499-546-HA-Puro

    Plasmid
    #193768
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1Δ499-546 (CDT1 Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193768-plasmid-map-sequence-id-380214

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  16. pADC10-SETXhel

    Plasmid
    #193056
    Purpose
    Baculovirus expression of human Senataxin helicase domain for protein production
    Depositor
    Andrew Deans
    Article
    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:
    Insert
    Senataxin (SETX Human)
    Tags
    Flag
    Expression
    Insect and Mammalian
    Mutation
    codon optimized for insect cell expression
    Promoter
    hybrid p10 (insect) and CMV (human) expression
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    193056-plasmid-map-sequence-id-381199

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  19. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

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Showing: 4361 - 4380 of 5133 results