163,486 results

1. pBlu-CGT-1

   Plasmid

   #170158

   Purpose

   Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

   Depositor

   Sarah MacDonald

   Insert

   Cyclomaltodextrin Glucanotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3536

   Plasmid

   #145012

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXD4 (HOXD4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPgiCas13b

   Plasmid

   #184833

   Purpose

   Inducible expression of PgiCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Porphyromonas gingivalis
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMBP-HRV3C-AcrIF9

   Plasmid

   #141442

   Purpose

   Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

   Depositor

   Blake Wiedenheft

   Article

   Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

   Insert

   AcrIF9
   

   Use

   Tags

   HRV3C cleavage site and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. BB2_AB

   Plasmid

   #98534

   Purpose

   empty BB2 for transcription unit cloning

   Depositor

   Diethard Mattanovich, Brigitte Gasser, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_EGFR_p.G719D

   Plasmid

   #81708

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G719D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiTet-LDLR-mCherry BlastR

    Plasmid

    #186739

    Purpose

    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    LDLR (LDLR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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