161,398 results

1. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-tevopreq1-GG-acceptor

   Plasmid

   #174038

   Purpose

   Prime editing in mammalian cells

   Depositor

   David Liu

   Article

   Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PEmax

   Plasmid

   #174820

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-AAVS1-5-1kbdonor

   Plasmid

   #234735

   Purpose

   PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   AAVS1-5 crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-NT*-HRV3CP

   Plasmid

   #162795

   Purpose

   Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

   Depositor

   Gottfried Otting

   Article

   Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

   Insert

   Human rhinovirus 14 3C protease
   

   Use

   Tags

   NT* solubility tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mini-pHelper-1.0

   Plasmid

   #230933

   Purpose

   The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

   Depositor

   Qizhao Wang

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCmini-iCAP-PHP.eB

   Plasmid

   #103005

   Purpose

   non-standard AAV2 rep-AAV-PHP.eB cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   Synthetic construct isolate AAV-PHP.eB VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBQ:RUBY

   Plasmid

   #160909

   Purpose

   RUBY under the control of Ubiquitin promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and UBIQUITIN promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHage2-EF1a-TERT-IRES-Puro

   Plasmid

   #182590

   Purpose

   expresses TERT in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Calreticulin-N-16

    Plasmid

    #55006

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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