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pMV261-Cas12a

Plasmid

#158707

Purpose

expresses codon-optimized FnCpf1

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

FnCpf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pnGFP

Plasmid

#64913

Purpose

Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Insert

pnGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pKEW107-MbCas12a-33362

Plasmid

#115654

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW106-MbCas12a-58069

Plasmid

#115653

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW321-MbCas12a-RFP-GFP-array

Plasmid

#115661

Purpose

Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

GFP and RFP array for MbCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro

Plasmid

#210855

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

V44D/V314D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V314D-mCherry-Hygro

Plasmid

#210854

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

V314D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V44D-mCherry-Hygro

Plasmid

#210853

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

V44D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG101

Plasmid

#210858

Purpose

Mammalian expression of ATG101

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG101 (ATG101 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

Plasmid

#210842

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCF738

Plasmid

#122032

Purpose

EF1a-WNV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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MazF-WT

Plasmid

#155202

Purpose

To express recombinant MazF protein.

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Insert

MazF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-VPS15-TSF

Plasmid

#203563

Purpose

Mammalian expression of VPS15 with TwinStrep-Flag tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

VPS15 (PIK3R4 Human)

Use

Tags

TwinStrep Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCF221

Plasmid

#121669

Purpose

U6-sgRNA EF1a-mCherry

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAC-crRNA-Cm

Plasmid

#158711

Purpose

Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

eforCP (for crRNA cloning)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

TetO

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ULK1 (ULK1 Human)

Use

Tags

MBP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7 RNAP

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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