We narrowed to 19,591 results for: ATO

1. pATK (pKD-G9)

   Plasmid

   #62688

   Purpose

   Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

   Insert

   tdTomato/amiR-eGFP123/amiR-eGFP419
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-15b PAI-RBP

   Plasmid

   #45390

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. bPAC-mycHis_pGEM

   Plasmid

   #85468

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp.

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   Bacterial Photoactivated Adenylyl Cyclase
   

   Use

   Tags

   His and Myc

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBER

   Plasmid

   #62662

   Purpose

   Donor vector for tdTomato knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Inserts

   tdTomato
   hygromycin
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B0015_DE

   Plasmid

   #66035

   Purpose

   MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Double Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. B0015_DF

   Plasmid

   #66036

   Purpose

   MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Double Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. B0015_DG

   Plasmid

   #66037

   Purpose

   MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:G]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Double Terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B0015_DH

    Plasmid

    #66038

    Purpose

    MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:H]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Double Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-20b-T7-Ntx

    Plasmid

    #58714

    Purpose

    Expresses T7-Notexin in Escherichia coli

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pINDUCER21-MYB

    Plasmid

    #51305

    Purpose

    Expression of MYB after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-myb avian myeloblastosis viral oncogene homolog (MYB Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINDUCER21-RORA

    Plasmid

    #51303

    Purpose

    Expression of RORA after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    RAR-related orphan receptor A (RORA Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCK351

    Plasmid

    #129696

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pINDUCER21-HOXA9

    Plasmid

    #51302

    Purpose

    Expression of HOXA9 after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    homeobox A9 (HOXA9 Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    n/a

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSFDuet-1 IntS8 AA 1-308

    Plasmid

    #196905

    Purpose

    Expresses His-tagged Drosophila IntS8 amino acids 1-308

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 8 (IntS8 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCK354

    Plasmid

    #129698

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120010799
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

    Plasmid

    #114378

    Purpose

    expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-KA2N-EGFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EGFP and KA2N

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid

    #141108

    Purpose

    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

    Depositor

    Matthew Gibson

    Article

    Hill et al Methods Mol Biol. 2022;2450:437-465. doi

    Insert

    Nematostella codon optimized Cas9
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    All amino acids coded for by codons optimized for…

    Promoter

    T7

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEMHE:mTREK-1(K271Q)

    Plasmid

    #133270

    Purpose

    X. laevis expression vector. It will generate the mouse TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    K271Q

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-MBD

    Plasmid

    #114371

    Purpose

    expresses Flpo recombinase-dependent GtACR2-MBD, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-MBD (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and MBD

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

    Plasmid

    #114374

    Purpose

    expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTE5419_p3WJdB-glcO36

    Plasmid

    #221193

    Purpose

    GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO36
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter with downstream glcO36 operator

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCK353

    Plasmid

    #129697

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCK355

    Plasmid

    #129699

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    ilvBN terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

    Plasmid

    #114373

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv4.2LL (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv4.2LL

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

    Plasmid

    #114375

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv2.1C

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

    Plasmid

    #114372

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Nlgn1C (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Nlgn1C

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pINDUCER21-SOX4

    Plasmid

    #51304

    Purpose

    Expression of SOX4 after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    SRY (sex determining region Y)-box 4 (SOX4 Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSFDuet-1 IntS6 AA 1035-1284

    Plasmid

    #196904

    Purpose

    Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 6 (IntS6 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSFDuet-1 IntS11 AA 300-597

    Plasmid

    #199329

    Purpose

    Expresses His-tagged Drosophila IntS11 amino acids 300-597

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 11 (IntS11 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 300-597 only

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

    Plasmid

    #114376

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP, Kv2.1C, and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEMHE:mTREK-1(G137I)

    Plasmid

    #133272

    Purpose

    X. laevis expression vector. It will generate the mouse TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    G137I

    Promoter

    T7

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMtnA FLAG-IntS12 puro

    Plasmid

    #195077

    Purpose

    Expresses FLAG-tagged Drosophila IntS12 from inducible MtnA promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator 12 (IntS12 Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-20b-T7-Ntx-K49

    Plasmid

    #58715

    Purpose

    Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    T7 peptide tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic Acid 49 to Lysine

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pINDUCER21-ERG

    Plasmid

    #51301

    Purpose

    Expression of ERG after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HR180-LGR5-iCT

    Plasmid

    #129094

    Purpose

    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC

    Plasmid

    #114377

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targeting

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv2.1C-linker-TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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41. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid

    #184562

    Purpose

    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines.

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pUb FLAG-Human IntS6L

    Plasmid

    #198409

    Purpose

    Expresses FLAG-tagged human IntS6L

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator complex subunit 6 like (INTS6L Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pIRES:mTREK-1(G137I)

    Plasmid

    #133274

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G137I

    Promoter

    CMVd2

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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44. pIRES:mTREK-1(K271Q)

    Plasmid

    #133273

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian and Yeast

    Mutation

    K271Q

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGG-PIP32

    Plasmid

    #196770

    Purpose

    Contains RBOHF promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    RESPIRATORY BURST OXIDASE HOMOLOG F (RBOH F Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGEMHE:mTREK-1_cryst

    Plasmid

    #133271

    Purpose

    X. laevis expression vector. It will generate the TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMXS-IRES-BLAST TFR1

    Plasmid

    #169890

    Purpose

    Express TFR1 open reading frame. Lacks IRE regulatory elements

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    TFRC (TFRC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    TFRC cDNA, lacking 3'UTR regulatory elements

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pPICZ:mTREK-1_cryst

    Plasmid

    #133269

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #69138

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSPL3-hRPH3A_c.444Gwt

    Plasmid

    #190476

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.444 [exon 7 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge