Addgene

We narrowed to 1,934 results for: Endog

Showing: 421 - 440 of 1934 results

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pcrRNA.ind

Plasmid

#61284

Purpose

Arabinose-inducible repeat-only E. coli CRISPR array

Depositor

Chase Beisel

Article

Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pChlr-3CQ

Plasmid

#52147

Purpose

Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 97-132 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-1SQ

Plasmid

#52139

Purpose

Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 25-60 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-2NQ

Plasmid

#52145

Purpose

Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 61-96 (PUM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Use

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pU1A-myc-T2A-tagRFP

Plasmid

#140269

Purpose

Expresses truncated U1A in mammalian cells

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Truncated U1A

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-Venus

Plasmid

#138388

Purpose

RMCE vector for generating C-terminal Venus fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

Venus

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSec7-iGFPx6

Plasmid

#115424

Purpose

Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-iGFPx6

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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Psicheck2 Sox4 WT 3'UTR oligo

Plasmid

#26990

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 wt UTR oligo (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

bp 449-509 of SOX4 3' UTR

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pSA109

Plasmid

#59783

Purpose

drives ZIF-1 protein in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
zif-1 coding sequence (zif-1 Nematode)
unc-54 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Psicheck2 UBE2f full length 3'UTR

Plasmid

#26991

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

UBE2f Full length 3' UTR (UBE2F Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
MAC_N_VPS26C

Plasmid

#209689

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_VPS26C

Plasmid

#209690

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_VPS35L

Plasmid

#209692

Purpose

Expresses MAC-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NC_000016.9:g.19710824G>A

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Zeo

Plasmid

#207734

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-miRFP670nano3-Zeo

Plasmid

#207735

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-3xFLAG-Zeo

Plasmid

#207739

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-V5-Blast

Plasmid

#207719

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge