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Plasmid#122565PurposeVector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only
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pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9A V266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 HuR.wt IRES Puro
Plasmid#110386Purposeγ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3
Plasmid#90087PurposeExpresses wild type caspase 3 in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationWild typePromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-FoxG1
Plasmid#35547DepositorInsertFoxG1 (Foxg1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pELPS-eDHFR-YFP-T2A-Renilla
Plasmid#193253PurposeTriple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferaseDepositorInsertThe eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
UseLentiviralTagsRenilla luciferase (rLuc) and yellow fluorescent …ExpressionMutationNonePromoterEF1aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-CeeIF4G_315-491opt_AB
Plasmid#148349PurposeBacterial Expression of CeeIF4G_315-491optDepositorInsertCeeIF4G_315-491opt (ifg-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625PurposeTet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIREShygro HA-mVenus-mAID
Plasmid#117714PurposeExpression of HA-tagged mVenus fused to mAIDDepositorInsertmVenus-mAID
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-40PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
UseTagsHis tagExpressionBacterialMutationdeleted amino acid 1-21PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Ld-VN
Plasmid#135520PurposeMammalian expression of VN-fused and myc-tagged H2-LdDepositorInsertH2-Ld
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.DelHNS IRES Puro
Plasmid#110389Purposeγ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationHNS (HuR nuclear-cytoplasmic shuttling sequence) …PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57
Plasmid#135516PurposeMammalian expression of myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
Plasmid#162037PurposeBackground-free mRNA labeling tripartite superfolder GFP systemDepositorInsertGFP1-9 P2A MCP-GFP10 P2A PCP-GFP11
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only