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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 159,238 results for: addgene

Showing: 421 - 440 of 159238 results

  1. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S52D
    Promoter
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21809-plasmid-map-sequence-id-358561

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  3. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Use
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  5. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Use
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

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  6. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  7. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pKG141

    Plasmid
    #69759
    Purpose
    Retroviral expression of CENP-B
    Depositor
    Iain Cheeseman
    Insert
    CENP-B (CENPB Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  10. CMV-dCas9_puroR

    Plasmid
    #219825
    Purpose
    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    CMV
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219825-plasmid-map-sequence-id-441124

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  11. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid
    #32522
    Depositor
    Mustafa Sahin
    Insert
    dominant negative Rheb (D60K) (Rheb Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D60K to make dominant negative
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. SLX4

    Plasmid
    #73242
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLX4 (SLX4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73242-plasmid-map-sequence-id-136207

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  13. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  14. Lentiviral sgRNA human CD19

    Plasmid
    #155289
    Purpose
    Lentiviral expression of sgRNA against human CD19
    Depositor
    Martin Carroll
    Insert
    CD19 (CD19 Human)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155289-plasmid-map-sequence-id-309995

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  15. 6N31

    Plasmid
    #127293
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR12 (WDR12 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    June 18, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127293-plasmid-map-sequence-id-247053

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  16. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Gary Yellen
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86920-plasmid-map-sequence-id-172992

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  17. PP-HSP90 (3O6O)

    Plasmid
    #28153
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HSP90
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. SHC1

    Plasmid
    #73249
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SHC1 (SHC1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73249-plasmid-map-sequence-id-136213

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  19. PP-HSP90 (3PEH)

    Plasmid
    #28267
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90 (PFL1070c Plasmodium Falciparum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains N-terminal domain.
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pSBtet-RB-PE2

    Plasmid
    #173903
    Purpose
    SB-transposon with inducible expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173903-plasmid-map-sequence-id-346343

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Showing: 421 - 440 of 159238 results