Addgene

We narrowed to 4,219 results for: biorxiv

Showing: 421 - 440 of 4219 results

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pBEST-PrOR1OR2-MGapt-UTR1-deGFP

Plasmid

#227648

Purpose

PrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PTet-MGapt-UTR1-deGFP

Plasmid

#227647

Purpose

pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'omega

Plasmid

#223870

Purpose

4G-cloning donor for 3'-Gibson overhang, omega-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'omega sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-TEV

Plasmid

#223872

Purpose

4G-cloning donor for N-terminal His6-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-3C

Plasmid

#223873

Purpose

4G-cloning donor for N-terminal His6-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-3C

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3

Plasmid

#223874

Purpose

4G-cloning donor for N-terminal His6-FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_ph

Plasmid

#223857

Purpose

4G-cloning donor for insect promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMV

Plasmid

#223860

Purpose

4G-cloning donor for mammalian promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_T7

Plasmid

#223854

Purpose

4G-cloning donor for bacterial promoter and terminator, no tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

T7 promoter and terminator

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-SUMO

Plasmid

#223879

Purpose

4G-cloning donor for N-terminal His6-SUMO tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-SUMO

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-TEV

Plasmid

#223881

Purpose

4G-cloning donor for N-terminal GFP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

GFP-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_TS-3C

Plasmid

#223883

Purpose

4G-cloning donor for N-terminal TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

TwinStrep-3C

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-3C

Plasmid

#223882

Purpose

4G-cloning donor for N-terminal GFP-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

GFP-3C

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-GFP

Plasmid

#223888

Purpose

4G-cloning donor for C-terminal 3C-GFP tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-GFP

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-His8

Plasmid

#223887

Purpose

4G-cloning donor for C-terminal 3C-His8 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-His8

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_CPD-His10

Plasmid

#223890

Purpose

4G-cloning donor for C-terminal CPD-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CPD-His10

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS

Plasmid

#223892

Purpose

4G-cloning donor for C-terminal 3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-TwinStrep

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'alpha

Plasmid

#223863

Purpose

4G-cloning donor for 5'-Gibson overhang, alpha-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'alpha sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'gamma

Plasmid

#223865

Purpose

4G-cloning donor for 5'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'gamma sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge