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tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE Tight

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMax_BFP_A191G

Plasmid

#177823

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP_A191G

Use

Tags

Expression

Mammalian

Mutation

A191G

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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proE-cad670-Luc

Plasmid

#42083

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only

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pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

Plasmid

#174884

Purpose

Targeting vector for generating Sox2 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Sox2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.Flag.HERC2-HECT-WT.6xHis

Plasmid

#39227

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pLuc-CDS-m215-2

Plasmid

#42102

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSV CDK9 HA (P#598)

Plasmid

#14638

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 (CDK9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag (-4)

Plasmid

#40914

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, and lacks the final fo…

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

Plasmid

#238185

Purpose

Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

KLF4 (KLF4 Synthetic, Human)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

Plasmid

#238181

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

KLF5 (KLF5 Synthetic, Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

Plasmid

#238184

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

KLF5 (KLF5 Synthetic, Human)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

Plasmid

#238167

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

Plasmid

#238182

Purpose

Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

Insert

KLF4 (KLF4 Synthetic, Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174881

Purpose

Targeting vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Flnc

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174885

Purpose

Targeting vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Usp5

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174886

Purpose

Targeting vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Wnk1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro_BC4

Plasmid

#193146

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CAAC

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro_BC23

Plasmid

#193165

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CTAG

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PB-SRT-Puro_BC22

Plasmid

#193164

Purpose

Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

Depositor

Rob Mitra

Article

Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

Insert

Barcoded piggyBac Puro SRT

Use

Tags

Expression

Mammalian

Mutation

Barcode: CAGT

Promoter

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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