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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CA

Plasmid

#69616

Purpose

To express gene strongly with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry_SeV_Nucleoprotein

Plasmid

#228806

Purpose

Expression of Sendai virus Nucleoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Nucleoprotein

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_SeV_RdRP

Plasmid

#228808

Purpose

Expression of Sendai virus RdRP

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

RdRP

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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piRFP_SeV_Phosphoprotein

Plasmid

#228807

Purpose

Expression of Sendai virus Phosphoprotein

Depositor

Shravanti Rampalli

Article

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

Phosphoprotein

Tags

iRFP670

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSCupSup35GFP

Plasmid

#1087

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

HIS tag

Expression

Bacterial

Available Since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-NbALFA-degFusionRed

Plasmid

#199466

Purpose

Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

NbALFA-degFusionRed

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smurf2 wt

Plasmid

#13504

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-D661A

Plasmid

#92236

Purpose

Express D661A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-D661A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Aspartate 661 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698N

Plasmid

#92242

Purpose

Express H698N mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698N mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Asparagine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Vinculin

Plasmid

#80024

Purpose

Mammalian expression of mCherry-vinculin

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
Flag-Gsdmd-NT C192A

Plasmid

#133891

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C39A

Plasmid

#133887

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Mutation

C39A

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C265A

Plasmid

#133892

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C77A

Plasmid

#133889

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available Since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 uGFP

Plasmid

#14557

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only