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Showing: 421 - 440 of 4788 results

  8. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Article
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  19. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  20. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid
    #138524
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    PRC2-binding G-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138524-plasmid-map-sequence-id-292291

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Showing: 421 - 440 of 4788 results