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pHAGE2-TetO-GFP-mSox17

Plasmid

#206373

Purpose

Expresses EGFP fused mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNTI827

Plasmid

#216635

Purpose

Cal-ID-ERM (V5) ER membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-IL3-218-PDGFR

Plasmid

#139055

Purpose

A lentivirus vector to integrate a surface membrane anchored human IL3 gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

interleukin 3(IL3) (IL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-2A-G(N2c)-WPRE

Plasmid

#194352

Purpose

Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

Depositor

Marco Tripodi

Article

Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Rabies CVS-N2c glycoprotein (G)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2 GFP-Atl1

Plasmid

#86679

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS/ES-EnvA-VSVg-WPRE-pA

Plasmid

#87221

Purpose

to package wild type EnvA psudotyped lentivirus

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

EnvA-VSVg

Use

Mouse Targeting and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNTI823

Plasmid

#216632

Purpose

Cal-ID variant with T195/G196 split site, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID (split site variant)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI828

Plasmid

#216636

Purpose

cpTurboID-ERM (V5) ER membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI935

Plasmid

#216638

Purpose

cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mSox17-3xflag

Plasmid

#206377

Purpose

Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-CSF2-218-PDGFR

Plasmid

#139058

Purpose

A lentivirus vector to integrate a surface membrane anchored human CSF2 gene

Depositor

Tao Liu

Article

Liu et al Cell Chem Biol. 2017 Jun 22;24(6):767-77

Insert

colony stimulating factor 2 (CSF2) (CSF2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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IY601: pMVP/Lenti/Blast-DEST

Plasmid

#121849

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ACTIN-SOX2-PGK Cre

Plasmid

#59019

Purpose

Bicistronic Lentivirus expressing sox2 and Cre

Depositor

Trudy Oliver

Article

Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

B-actin

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #1 GFP

Plasmid

#106950

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

Plasmid

#114212

Purpose

Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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SiC-V1-Scr

Plasmid

#133042

Purpose

SiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(R163A)-Hygro

Plasmid

#187609

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

Depositor

Robert Sobol

Article

Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

Insert

LivePAR(R163A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

R163A mutation in the WWE Domain

Promoter

EF1A

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Plasmid

#125230

Purpose

For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

Use

Lentiviral

Tags

HA,Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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