We narrowed to 1,322 results for: pET28a

1. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-CPL2/pET28a

   Plasmid

   #172095

   Purpose

   cDNA of CER11/CPL2 cloned into pET28a for protein expression

   Depositor

   George Haughn

   Article

   Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

   Insert

   CPL2 (CPL2 Mustard Weed)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28a-XPO5-His6

   Plasmid

   #171562

   Purpose

   Encodes human XPO5 protein

   Depositor

   Yan Zeng

   Insert

   XPO5 (XPO5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a SznF E281A

   Plasmid

   #139591

   Purpose

   expresses SznF E281A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E281A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 281 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a SznF H225A

   Plasmid

   #139590

   Purpose

   expresses SznF H225A

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H225A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed histidine 225 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SznF E215A

   Plasmid

   #139565

   Purpose

   expresses SznF E215A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF E215A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-DDX43-NT-G46D

   Plasmid

   #134588

   Purpose

   Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G154D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-DDX43-FL-G84D

   Plasmid

   #134577

   Purpose

   Express human DDX43 mutant (full-length with G84D mutation) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43-G84D (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length (G84D)

   Promoter

   T7

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-clbH-C-A2-PCP

   Plasmid

   #114157

   Purpose

   Expresses ClbH-C-A2-PCP

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Use

   Tags

   6xHis-thrombin-T7 tag

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH

   Promoter

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_6HT-TBP6.7(P46A)

    Plasmid

    #112721

    Purpose

    This mutation (P46A) marks the first residue within the evolved region of U1A.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P46A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P46A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_6HT-TBP6.7(Q48T)

    Plasmid

    #112725

    Purpose

    This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48T)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48T

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_6HT-TBP6.7(P51A)

    Plasmid

    #112728

    Purpose

    Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(P51A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    P51A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(Q54A)

    Plasmid

    #112730

    Purpose

    Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q54A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q54A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-RupA_T1-NHis

    Plasmid

    #109259

    Purpose

    NRPS T domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_T1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 917..1025 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-RupA_R-NHis

    Plasmid

    #109257

    Purpose

    NRPS R domain with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Schneider et al Tetrahedron

    Insert

    RupA_R
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    contains aa 2038..2443 of WP_015523502.1

    Promoter

    T7

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge