We narrowed to 2,326 results for: PUC19

1. pNOC_hfnCas12a-Nlux_crRNA NR3

   Plasmid

   #176246

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC_hfnCas12a_crRNA NR3

   Plasmid

   #176249

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR and Synthetic Biology; Expression in micro…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ir7f-T2A-QF2 HDR plasmid

   Plasmid

   #140942

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   4 point mutations (annotated on plasmid map) were…

   Promoter

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HBT-CPK10ac-Flag

   Plasmid

   #107427

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HBT-CPK10-Flag

   Plasmid

   #107428

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK10 (CDPK1 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. HBT-CPK30ac-Flag

   Plasmid

   #107429

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HBT-CPK30-Flag

   Plasmid

   #107430

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK30 (CPK30 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HBT-CPK32ac-Flag

   Plasmid

   #107431

   Purpose

   For Arabidopsis mesophyll protoplast transient expression assay

   Depositor

   Jen Sheen

   Article

   Liu et al Nature. 2017 May 18;545(7654):311-316. d

   Insert

   CPK32 (CPK32 Mustard Weed)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ID1GFP Donor

   Plasmid

   #119282

   Purpose

   Mouse ID1-eGFP for HR to create reporter

   Depositor

   Joan Massague

   Article

   Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

   Insert

   Id1GFP + homology arms (Id1 Mouse)
   

   Use

   Reporter

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Empty Bridge Control Zfp462-Klf4SE

    Plasmid

    #127666

    Purpose

    Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    gRNAs 129, 135, 115, 117
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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