We narrowed to 11,726 results for: pcdna

1. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

   Plasmid

   #140979

   Purpose

   Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-C-8His-Flag

   Plasmid

   #157678

   Purpose

   C-terminal tagging vector

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   8His-Flag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5/FRT/TO GFP DNAJB9

   Plasmid

   #19505

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB9 (DNAJB9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Ikkb-FLAG(K44A)

   Plasmid

   #23299

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PERK 3: PERK.dC.9E10.pCDNA

   Plasmid

   #21816

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (C-term deleted) (Eif2ak3 Mouse)
   

   Use

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Mutation

   amino-acids 582-1081 deleted

   Promoter

   Available since

   July 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CD9-(TEV)-tTA

   Plasmid

   #162596

   Purpose

   human CD9-TEV cleavage site-tTA

   Depositor

   Masaharu Somiya

   Article

   Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

   Insert

   human CD9 (CD9 Human)
   

   Use

   Tags

   TEV protease cleavage site and tTA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyso-PTEN(A4)-mCherry

   Plasmid

   #184054

   Purpose

   Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-PTEN(A4)-mCherry (PTEN Human)
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1). a…

   Expression

   Mammalian

   Mutation

   Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT-ChemoD-NAD

   Plasmid

   #193826

   Purpose

   CMV overexpression of ChemoD-NAD in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoD-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ShadowG: T225R, HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA8-Flag-LARS(1-976aa)

    Plasmid

    #139689

    Purpose

    Expresses N-teminal Flag tagged aa 1-976 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-FLAG-SBP-STAG2 (WT)

    Plasmid

    #73963

    Purpose

    STAG2 cDNA (CCDS43990) wild type

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3puro-EGFP-FXR1-isoform b

    Plasmid

    #225449

    Purpose

    Express EGFP-fusion protein of FXR1 isoform b

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform b (FXR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. RelA RHD cFlag pcDNA3

    Plasmid

    #20015

    Depositor

    Stephen Smale

    Insert

    RelA (Rela Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Contains only the Rel Homology Domain of RelA. It…

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-Y1214F-VEGFR2-mCherry

    Plasmid

    #139609

    Purpose

    Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187922

    Purpose

    Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase acidic protein
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-2xFLAG-2xSTREP-CCND1

    Plasmid

    #172648

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. 1481 pcDNA3 flag HA 14-3-3 zeta

    Plasmid

    #9002

    Depositor

    William Sellers

    Insert

    14-3-3 zeta (YWHAZ Bovine)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    T226A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-Tet3 CD Mut

    Plasmid

    #72222

    Purpose

    Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 3
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET3 catalytic domain (cata…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only