We narrowed to 2,485 results for: organization Addgene

1. Lenti-CMV-MLH1dn-IRES-puroR

   Plasmid

   #207354

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   MutL homolog 1 (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Δ754-756 dominant negative mutant

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGL4.11-COL1A1mSMAD

   Plasmid

   #230917

   Purpose

   Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant potential SMAD bindin…

   Promoter

   COL1A1

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

   Plasmid

   #207357

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

   Plasmid

   #239102

   Purpose

   Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

   Depositor

   Karl Wahlin

   Article

   Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

   Insert

   POU4F2 (POU4F2 Human)
   

   Use

   Donor plasmid for integration into the human geno…

   Tags

   p2A-tdTomato-p2A-Thy1.2

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous POU4F2

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. P530_SIX6-p2A-h2b-EGFP

   Plasmid

   #239101

   Purpose

   Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

   Depositor

   Karl Wahlin

   Article

   Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

   Insert

   SIX6 (SIX6 Human)
   

   Use

   Donor plasmid

   Tags

   p2A-h2b-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous SIX6 promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGL4.11-COL1A1mTEAD

   Plasmid

   #230916

   Purpose

   Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase- luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   mutant potential TEAD bind elements AGGAAT to CTG…

   Promoter

   COL1A1

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIG1a_StrepII-Hook3_FTS_FHIP1B

   Plasmid

   #222299

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3 (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

   Plasmid

   #222300

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[451-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

   Plasmid

   #222301

   Purpose

   Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK3[571-718] (HOOK3 Human)
   FTS (AKTIP Human)
   FHIP1B (FHIP1B Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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