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pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159814

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti Dest PuroR R1-R2

Plasmid

#84575

Purpose

Gateway destination vector expressing Puromycin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-puro

Plasmid

#189992

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

Plasmid

#211845

Purpose

Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Inserts

LRR-NEL
GAL4-VP16-GR

Use

Synthetic Biology

Tags

HA, Myc, and mCherry

Expression

Plant

Mutation

C492A

Promoter

6xUAS-35Smini and pUBQ10

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

Plasmid

#222634

Purpose

Rho reporter, sensor dead

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor (PKN-L59Q)

Use

Lentiviral

Tags

Expression

Mutation

PKN L59Q

Promoter

EF1a

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(WT)

Plasmid

#140729

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSC5GGv2

Plasmid

#188604

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pST_DSUP_RAMVA (pBS0353)

Plasmid

#185189

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mTcte1-PA

Plasmid

#176469

Purpose

Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

t-complex-associated testis expressed 1 (Tcte1 Mouse)

Use

Tags

PA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-N-mSOG-FRB-IRES-mCherry

Plasmid

#136602

Purpose

Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

N-mSOG-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p37-Strep-HA

Plasmid

#113485

Purpose

Expression of human p37 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p37 (UBXN2B Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A-3xFlag_IRES-GFP

Plasmid

#198390

Purpose

RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-Omp25

Plasmid

#38249

Depositor

Noboru Mizushima

Article

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

plasmid contains AA109-145 of rat Omp25/Synj2bp

Promoter

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mScarlet-H2BC11

Plasmid

#207756

Purpose

Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_N_SOX2

Plasmid

#167794

Purpose

Expresses SOX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

SOX2 (SOX2 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEX-GFP-hLC3ΔG

Plasmid

#24988

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Available since

June 28, 2010

Availability

Academic Institutions and Nonprofits only