We narrowed to 13,117 results for: CRISPR-Cas9

1. pUDP012

   Plasmid

   #101167

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUDP044

   Plasmid

   #101168

   Purpose

   pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EC67843

   Plasmid

   #209513

   Purpose

   Cloning in complete guide expression cassettes, with Cas12a (D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAW005-2

   Plasmid

   #85611

   Purpose

   Empty gRNA delivery vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EC67841

   Plasmid

   #209512

   Purpose

   Cloning in complete guide expression cassettes, with Cas12a (D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EC67842

   Plasmid

   #209516

   Purpose

   Cloning in complete guide expression cassettes, with Cas12a (D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXZ13lacZalpha

   Plasmid

   #160229

   Purpose

   places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSPneoHHgRNAH

   Plasmid

   #63557

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX20

   Plasmid

   #169443

   Purpose

   Used for guide cloning with PCR

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX52

    Plasmid

    #169444

    Purpose

    Used for guide cloning with PCR

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSPneogRNAH

    Plasmid

    #63556

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMLS257

    Plasmid

    #73716

    Purpose

    SapTrap destination vector for building repair template only vectors

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    SapI repair template insertion site
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS272

    Plasmid

    #73728

    Purpose

    SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    syntron embedded C-terminal FLP-on
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMLS281

    Plasmid

    #73732

    Purpose

    SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    syntron embedded N-terminal FLP-on
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMLS285

    Plasmid

    #73734

    Purpose

    SapTrap egl-13 NLS connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    egl-13 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psLIB TRE mito-mCherry

    Plasmid

    #229966

    Purpose

    encodes a mCherry targeted to mitochondria under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mito-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

    Plasmid

    #199490

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

    Plasmid

    #199491

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7

    Plasmid

    #199492

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP11x7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKozak_mTagBFP2-T2A-sGFP1-10_synCoTC

    Plasmid

    #199494

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge