We narrowed to 17,694 results for: Comp;

1. pOPINK-UBE2S

   Plasmid

   #66714

   Purpose

   Expresses UBE2S (1-196) in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Insert

   e2-epf (UBE2S )
   

   Use

   Tags

   GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCTcon2-5INS_EV3

   Plasmid

   #102293

   Purpose

   Yeast Surface Display of 5Ins_16_EV3

   Depositor

   Sarel Fleishman

   Article

   Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

   Insert

   Evolved Insulin binding scFV
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJZC603

   Plasmid

   #62317

   Purpose

   sgRNA with 2x PP7 for yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA + 2x PP7 RNA binding module
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOTv6-puro-3Myc:mScarletI:3Myc

   Plasmid

   #179804

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mScarlet-I
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGG024

   Plasmid

   #113991

   Purpose

   Level 1 golden gate cloning vector for medium copy number free-living expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGagMBPHis

   Plasmid

   #42176

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   Gag protein sequence is from HIV-1 clone NL4-3
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-postASAP-p2a-ChrimsonR

   Plasmid

   #178794

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and ChrimsonR
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFlox c-RAD21_FKBPF36V_EYFP_Bsd

   Plasmid

   #163871

   Purpose

   Acute Depletion of RD21-EYFP

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Rad21 (Rad21 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTurquoise2-PXN

    Plasmid

    #112957

    Purpose

    In vivo visualization of focal adhesions (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    PXN
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmNFM

    Plasmid

    #83126

    Purpose

    Mouse neurofilament protein M cDNA mammalian expression construct

    Depositor

    Anthony Brown

    Article

    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

    Insert

    mouse NFM (Nefm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAct::Raf

    Plasmid

    #11341

    Depositor

    Michael Wigler

    Article

    Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

    Insert

    byr-2 (byr2 Fission Yeast)
    

    Use

    Tags

    Gal4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

13. LiRT

    Plasmid

    #214075

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LiRTX_ctrl

    Plasmid

    #214077

    Purpose

    Luminescent reporter system, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCellFree_G01

    Plasmid

    #67135

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EB3-mTurquoise2

    Plasmid

    #98825

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    EB3
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDonorTp

    Plasmid

    #211788

    Purpose

    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    dhfr
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge