We narrowed to 18,681 results for: tec

1. pEBFP2-Nuc

   Plasmid

   #14893

   Purpose

   Mammalian expression of EBFP2 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pEBFP2-Nuc
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMET7-GAG-EGFP

   Plasmid

   #80605

   Purpose

   Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-SpCas9mSA-His

   Plasmid

   #182951

   Purpose

   Express SpCas9mSA in bacteria

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   NLS-SpCas9mSA-His
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. EK0396 BFPmut-stop-t70587 (pEAQ)

   Plasmid

   #191171

   Purpose

   EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   t70587 (plant)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-GRAB_ATP1.0mut

   Plasmid

   #167578

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-c-Fos-(GGS)X6-HaloTag C58

   Plasmid

   #171437

   Purpose

   Expression of c-Fos-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   c-Fos-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pS2513·PHP

   Plasmid

   #122590

   Purpose

   Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

   Depositor

   Pablo Ivan Nikel

   Article

   Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

   Insert

   phIorine2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pem7

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

   Plasmid

   #171438

   Purpose

   Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-c-Jun
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EBFP2-Mito-7

    Plasmid

    #55248

    Purpose

    Localization: Mitochondria, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pH-nCas9-PBE

    Plasmid

    #98163

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA284= pSin-Pur-MCS-Puro

    Plasmid

    #116877

    Purpose

    Lentiviral transfer vector containing a multible cloning site

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9 TLS1

    Plasmid

    #196977

    Purpose

    Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS1 tag
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CP1041-ABEmax

    Plasmid

    #119808

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1119 (pDonor)

    Plasmid

    #160732

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCBB-eGFP

    Plasmid

    #32548

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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