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pFS_0346_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1_RC

Plasmid

#116958

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0365_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_2

Plasmid

#116977

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0364_pET30-RT-CRISPR_Desulfarculus_baarsii_DSM_2075_ARRAY_1_RC

Plasmid

#116976

Purpose

Expresses DbRT-Cas1-Cas2 under pT7lac promoter, encodes DbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Desulfarculus baarsii DSM 2075 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0345_pET30-RT-CRISPR_Bacteroides_fragilis_strain_S14_ARRAY_1

Plasmid

#116957

Purpose

Expresses BfRT-Cas1-Cas2 under pT7lac promoter, encodes BfCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Bacteroides fragilis strain S14 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0377_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2

Plasmid

#116989

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0360_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_2_RC

Plasmid

#116972

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0349_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1

Plasmid

#116961

Purpose

Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC

Plasmid

#116962

Purpose

Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0378_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_2_RC

Plasmid

#116990

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0375_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1

Plasmid

#116987

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0392_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2_RC

Plasmid

#117004

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0391_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_2

Plasmid

#117003

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0390_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1_RC

Plasmid

#117002

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0389_pET30-RT-CRISPR_Eubacterium_saburreum_DSM_3986_ARRAY_1

Plasmid

#117001

Purpose

Expresses EsRT-Cas1-Cas2 under pT7lac promoter, encodes EsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Eubacterium saburreum RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC

Plasmid

#116986

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC

Plasmid

#116984

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1

Plasmid

#116985

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC

Plasmid

#116982

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2

Plasmid

#116983

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW218

Plasmid

#113240

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK26

Plasmid

#105429

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Bs3

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM16841

Plasmid

#105416

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

STOP codon and nos terminator

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG173

Plasmid

#105417

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mEGFP (GFP variant)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG172

Plasmid

#105418

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mTRQ (GFP variant)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG171

Plasmid

#105419

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

mVenus (GFP variant)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG021

Plasmid

#105420

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

hpt (hygromycin phosphotransferase)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG351

Plasmid

#105421

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

nptII (neomycin phosphotransferase II)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG031

Plasmid

#105422

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

AvrRps4 Pseudomonas syringae pv. pisi)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJOG213

Plasmid

#105385

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor SlEDS1 (Solyc060g071280; 1200bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge