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mTagBFP2-Annexin-12

Plasmid

#55276

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Annexin (A4) (ANXA4 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-28:TUBA1B-mTagRFP-T

Plasmid

#101785

Purpose

Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

Depositor

Allen Institute for Cell Science

Insert

TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)

Use

CRISPR; Donor template

Tags

mTagRFPT-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-FOXD3

Plasmid

#153118

Purpose

Expression of Flag tagged human FOXD3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD3 (FOXD3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF5 CTFdeltaTA-FLAG

Plasmid

#217731

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF5 (ADGRF5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-996

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG5 CTFdeltaTA-FLAG

Plasmid

#217736

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG5 (ADGRG5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-232

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF4 CTFdeltaTA-FLAG

Plasmid

#217730

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF4 (ADGRF4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-390

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-SOX3

Plasmid

#61546

Purpose

Expresses SOX3 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

Plasmid

#133801

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA4

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CD81-10

Plasmid

#55281

Purpose

Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD81 (CD81 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTagBFP2-H3.3-N-14

Plasmid

#55302

Purpose

Localization: Nucleus/Histones, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

H3.3 (H3f3b Mouse)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-FKBP-PI4KAC1001(D1957A)

Plasmid

#139312

Purpose

Recruitable PI4KA kinase-dead catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

D1957A, G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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DSCAM_pCSdest

Plasmid

#53906

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DSCAM (DSCAM Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

Plasmid

#133802

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA5

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-Tubulin-C-18

Plasmid

#54292

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

Insert

Tubulin (TUBA1B Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-FKBP-PI4KAC1001

Plasmid

#139311

Purpose

Recruitable PI4KA catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

Plasmid

#164038

Purpose

Expression of dCas9-14xGFP11 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

dCas9-14xGFP11

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

dSV40

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

Plasmid

#109320

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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ADAMTS1_pCSdest

Plasmid

#53807

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS1 (ADAMTS1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits