We narrowed to 6,654 results for: SIM;

1. pROF050

   Plasmid

   #155327

   Purpose

   Constitutive expression of BRD-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-BRD-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJAT58

   Plasmid

   #204304

   Purpose

   Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   3XP3

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAT71

   Plasmid

   #204305

   Purpose

   Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS506

   Plasmid

   #200712

   Purpose

   Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Insert

   PMA1 promoter (PMA1 Budding Yeast)
   

   Use

   Synthetic Biology; Modular cloning

   Tags

   Expression

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1

   Plasmid

   #202012

   Purpose

   Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

   Insert

   NbGP41-sfGFP-VP64-GB1
   

   Use

   Synthetic Biology; Moclo level 1 vector

   Tags

   GB1 and sfGFP

   Expression

   Mutation

   Promoter

   AtUBI10

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. His SUMO TEV R221C SPOP 28-359

   Plasmid

   #194116

   Purpose

   R221C His SUMO SPOP expression plasmid

   Depositor

   Tanja Mittag

   Article

   Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

   Insert

   SPOP R221C (SPOP Human)
   

   Use

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Mutation

   R221C

   Promoter

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2h70CRET(#325)

   Plasmid

   #184060

   Purpose

   cyclofen-inducible CRE activation in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

   Insert

   CRE-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Hsp70-4

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_YES1_SH3

   Plasmid

   #178492

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   YES1_SH3 (YES1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmGe1_944-1354-V5His6_D

   Plasmid

   #146136

   Purpose

   Insect Expression of DmGe1_944-1354

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmGe1_944-1354 (Ge-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmCup_C

    Plasmid

    #146064

    Purpose

    Insect Expression of DmCup

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmCup (cup Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-lambdaN-HA-DmMe31B_1-267_C

    Plasmid

    #146065

    Purpose

    Insect Expression of DmMe31B_1-267

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmMe31B_1-267 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmMe31B_268-459_C

    Plasmid

    #146066

    Purpose

    Insect Expression of DmMe31B_268-459

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmMe31B_268-459 (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmHPat_A

    Plasmid

    #145893

    Purpose

    Insect Expression of DmHPat

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmHPat (Patr-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent mutations compared to the sequence g…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1-Ck1-hs1-l-chlo

    Plasmid

    #163937

    Purpose

    For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha trnG_trnfM region left homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. L1-Ck4-hs1-r-chlo

    Plasmid

    #163940

    Purpose

    For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha trnG_trnfM region right homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. L1-Ck4-hs2-r-chlo

    Plasmid

    #163941

    Purpose

    For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha rbcL_trnR region right homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. PROM5_MpU6

    Plasmid

    #136120

    Purpose

    Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpU6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDS_Cas9-NLS

    Plasmid

    #136121

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_AtcoCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CDS12-MpER-Targ

    Plasmid

    #136094

    Purpose

    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    ER targeting peptide from predicted chitinase Mapoly0069s0092
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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