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pQCX-CD28 Y189F

Plasmid

#223641

Purpose

Retroviral expression of mouse CD28 (Y189F) in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT46

Plasmid

#218724

Purpose

Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT46 (IFT46 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT25

Plasmid

#218721

Purpose

Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT25 (HSPB11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Tags

ALFA tag

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 CDS A. thaliana MAMI

Plasmid

#209186

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT5G54110.1 (MAMI Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B1(E234Q)-HA

Plasmid

#209253

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Mutation

E234Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B1(E234Q)-HA

Plasmid

#209241

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Tags

HA

Expression

Mammalian

Mutation

E234Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D330, 332N IRES GFP

Plasmid

#195702

Purpose

Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D330, 332N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 BBS pMT2

Plasmid

#206062

Purpose

expression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549

Depositor

Annette Dolphin

Article

Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BR1-P2A-FusionRed

Plasmid

#192819

Purpose

Expresses BR1 with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW2-NgBR

Plasmid

#203165

Purpose

Yeast expression vector for hNUS1

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

NUS1 (NUS1 Human)

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (277-357)

Plasmid

#203434

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (116-439)

Plasmid

#203425

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (1-357)

Plasmid

#203429

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (201-298)

Plasmid

#203431

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (208-300)

Plasmid

#203432

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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