We narrowed to 24,466 results for: nes

1. tet-pLKO shMKP5.v1 puro

   Plasmid

   #87792

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX302 PCSK5-V5 puro

   Plasmid

   #98389

   Purpose

   Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-DIO-erHRP(N175S mutant)

   Plasmid

   #79906

   Purpose

   Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

   Depositor

   Joshua Sanes

   Article

   Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

   Insert

   erHRP(N175S mutant)
   

   Use

   AAV and Cre/Lox

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   N175S mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-CCL5.Pro

   Plasmid

   #79492

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CCL5 Promoter (CCL5 Synthetic, Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   CCL5 Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet-pLKO shMKP1.v2 puro

   Plasmid

   #87791

   Purpose

   Expresses an inducible short hairpin targeting human MKP1 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP1 (DUSP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG3_hph

   Plasmid

   #184919

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TT JunD-HA

   Plasmid

   #58498

   Purpose

   Gateway entry vector for an inducible HA-tagged mouse JunD

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JunD (Jund Mouse)
   

   Use

   Entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pVenus-CyclinB1(R42A) (1317)

    Plasmid

    #39873

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    CyclinB1(R42A) (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    R42A = destruction box mutations = non-destructib…

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

    Plasmid

    #39858

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    C11orf51 (ANAPC15 Human)
    

    Use

    Tags

    IRES2-mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid

    #92354

    Purpose

    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

    Plasmid

    #124870

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid

    #100963

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    F138I, T78H

    Promoter

    PMA

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

    Plasmid

    #19746

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 and PTEN (Pten Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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