We narrowed to 4,899 results for: phy

1. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCWU6-xylR-E-luciferase

   Plasmid

   #190721

   Purpose

   fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

   Plasmid

   #51094

   Purpose

   Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   oChIEF(E163A/T199C)
   

   Use

   AAV

   Tags

   P2A-dTomato

   Expression

   Mammalian

   Mutation

   E163A/T199C

   Promoter

   EF1a

   Available since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Butelase AY

   Plasmid

   #221847

   Purpose

   Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

   Insert

   Butelase 1 ligase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Val237Ala, Thr238Tyr

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

   Plasmid

   #51095

   Purpose

   Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   ChIEF(E162A/T198C)
   

   Use

   AAV

   Tags

   P2A-dTomato

   Expression

   Mammalian

   Mutation

   E162A/T198C

   Promoter

   EF1a

   Available since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV::ratSyGCaMP2

   Plasmid

   #26124

   Depositor

   Leon Lagnado

   Article

   Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

   Insert

   Synaptophysin GCaMP2 (Syp Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCWU6-xylR-E-lepB

   Plasmid

   #190725

   Purpose

   fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-lepB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA CRU HISMX

   Plasmid

   #131164

   Purpose

   PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

   Plasmid

   #184294

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Adrb1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CD44MMA-c002

    Plasmid

    #175452

    Purpose

    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.

    Depositor

    Opher Gileadi

    Insert

    Cd44 (Cd44 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

    Plasmid

    #184293

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

    Depositor

    Bernardo Sabatini

    Inserts

    sgRNA-Adra1b
    sgRNA-Adra1b
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSuccessor

    Plasmid

    #228391

    Purpose

    intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

    Insert

    pConst23100-sfGFP__pLambda-DHFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid

    #226590

    Purpose

    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

    Depositor

    D. James Surmeier

    Insert

    PSAML-141F,Y115F-GlyR
    

    Use

    AAV

    Tags

    P2A-Fusion Red

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Rab11A[8-175]-His-C Q70L

    Plasmid

    #228042

    Purpose

    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

    Depositor

    Wade Harper

    Insert

    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q70L

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FB376

    Plasmid

    #209129

    Purpose

    Multipartite assembly of the TU for YFP expression with the TU for the auxotrophy marker pyr4 from T. reseei

    Depositor

    Jose Marcos

    Article

    Ropero-Pérez et al Curr Res Biotechnol 2024

    Insert

    PgpdA:yfp:TtrpC::Ppyr4:pyr4:Tpyr4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

    Plasmid

    #184292

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Grp
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

    Plasmid

    #184291

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Oprl1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

    Plasmid

    #184290

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Rxfp3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184295

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EPB41L3A-c021

    Plasmid

    #175460

    Purpose

    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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