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Plasmid#62913PurposeAAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromotershort form of Elongation Factor1α (EFS)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmpT
Plasmid#112123PurposeBacterial expression outer membrane protein T (OmpT), wild typeDepositorInsertOmpT (ompT E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2381-Tier1-PhCMV-Igk_TM
Plasmid#169487PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).DepositorInsertPCMV-driven membrane localization
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB415
Plasmid#68680PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Peroxisomes-2
Plasmid#54804PurposeLocalization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCherry-m…ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b
Plasmid#52122Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
UseTagsExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagseGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1-mCherry-NKD2
Plasmid#176641PurposeExpresses NKD2-mCherry fusion protein in mammalian cellsDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB216
Plasmid#68607PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-PD-4xDQNAT
Plasmid#128399PurposePeriplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertH. influenzae protein D
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-PorA-4xDQNAT
Plasmid#128400PurposePeriplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertN. meningitidis PorA
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL3664
Plasmid#162240PurposeTranscribes E. coli SRP RNA sequence for SHAPE-SeqDepositorInsertsE. coli SRP RNA
HDV
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDB368
Plasmid#110276PurposegHH_44 with a N-terminal 10-His DsbC TEV-peptide fusionDepositorInsertgHH_44
UseTags10-His, Tev recognition peptide, and mature DsbC …ExpressionBacterialMutationPromoterT7Available sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Peroxisomes-2
Plasmid#54847PurposeLocalization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528DepositorTypeEmpty backboneUseTagsPA-mCherry-miniSOG and Peroximal Targeting Signal…ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
UseTagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_3xNLS
Plasmid#65673PurposeExpresses NLS-CNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_3xNLS
Plasmid#65672PurposeExpresses NLS-YNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His mICA NG
Plasmid#70162PurposeExpresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydraseDepositorInsertmouse inhibitor of carbonic anhydrase
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn470Asp and Asn645AspPromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-CKAR2
Plasmid#236097PurposeCytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertExRai-CKAR2-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only