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We narrowed to 11,800 results for: emb

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Showing: 4461 - 4480 of 11800 results

  4. pEABR NA 3C FLAG SA

    Plasmid
    #234987
    Purpose
    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EABR-NA
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234987-plasmid-map-sequence-id-468750

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  5. siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)

    Plasmid
    #226578
    Purpose
    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen
    Depositor
    Gia Voeltz
    Publication
    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382
    Insert
    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    F196A, L199A, F200A, L203A, Y206A, L210A, L214A, …
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    226578-plasmid-map-sequence-id-451909

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  6. pODN-mNG-RHOA

    Plasmid
    #183836
    Purpose
    Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RHOA homology arms with mNeonGreen-linker (RHOA Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183836-plasmid-map-sequence-id-362444

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  7. pTagGFP2-N SIN1 deltaCRIM

    Plasmid
    #124921
    Purpose
    For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)
    Depositor
    Jin Chen
    Publication
    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPKAP1 (MAPKAP1 Human)
    Tags
    TagGFP2
    Expression
    Mammalian
    Mutation
    Insert ORF was sirently mutated to be resistent t…
    Available since
    May 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    124921-plasmid-map-sequence-id-244576

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  8. pTagGFP2-N SIN1 deltaPH

    Plasmid
    #124922
    Purpose
    For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)
    Depositor
    Jin Chen
    Publication
    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPKAP1 (MAPKAP1 Human)
    Tags
    TagGFP2
    Expression
    Mammalian
    Mutation
    Insert ORF was sirently mutated to be resistent t…
    Available since
    May 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    124922-plasmid-map-sequence-id-244578

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  9. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pJL CusS NarX Chimera

    Plasmid
    #225652
    Purpose
    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    CusS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225652-plasmid-map-sequence-id-455039

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  11. pJL NrsS NarX Chimera

    Plasmid
    #225653
    Purpose
    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NrsS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225653-plasmid-map-sequence-id-454026

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  12. pJL VanS NarX Chimera

    Plasmid
    #225655
    Purpose
    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    VanS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225655-plasmid-map-sequence-id-454023

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  13. PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250913
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (SEC61B, ARFGAP1 Human)
    Tags
    EGFP on Sec61b and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    250913-plasmid-map-sequence-id-498650

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  16. pJL RssA NarX Chimera

    Plasmid
    #225654
    Purpose
    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    RssA-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225654-plasmid-map-sequence-id-454025

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  19. pRS405 PEX14-VAC8-RFP

    Plasmid
    #166844
    Purpose
    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF
    Depositor
    Daniel Klionsky
    Publication
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, PEX14 Budding Yeast)
    Tags
    PEX14 transmembrane domain and RFP
    Expression
    Yeast
    Mutation
    PEX14 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166844-plasmid-map-sequence-id-329332

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Showing: 4461 - 4480 of 11800 results