We narrowed to 5,625 results for: feng zhang

1. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-G88P7-rM3Ds-2A-EYFP

   Plasmid

   #213970

   Purpose

   AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   rM3Ds
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P7

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-GFAP-NLS-CasRx-NLS-FLAG

   Plasmid

   #154000

   Purpose

   Express CasRx for AAV package

   Depositor

   Hui Yang

   Article

   Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-G88P3-HA-hM3Dq

   Plasmid

   #213972

   Purpose

   AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P3

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

   Plasmid

   #194755

   Purpose

   Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Insert

   PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

   Plasmid

   #224388

   Purpose

   Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 7 domain deleted (aa 286-323)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

   Plasmid

   #224387

   Purpose

   Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 6 domain deleted (aa 242-282)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

   Plasmid

   #224385

   Purpose

   Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 4 domain deleted (aa 153-195)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_GNB1L_gRNA

   Plasmid

   #224377

   Purpose

   Targets GNB1L for dTAG C terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

    Plasmid

    #194753

    Purpose

    Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194752

    Purpose

    Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPRv2-sgCTL

    Plasmid

    #234771

    Purpose

    Non Targeting Control

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Non Targeting Control sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas12Max

    Plasmid

    #195334

    Purpose

    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized Cas12Max
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    N243R

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. xCas12i

    Plasmid

    #195333

    Purpose

    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized xCas12i
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh24

    Plasmid

    #106697

    Purpose

    expression of gRNA targeting POMT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POMT2 (POMT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

17. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid

    #196727

    Purpose

    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge