We narrowed to 4,942 results for: puc
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Plasmid#232727PurposeA dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).DepositorInsertpegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScr-IFl_WT-IFr
Plasmid#192208PurposeScrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertScrambled I-F Leader and wildtype I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IFr
Plasmid#192209PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-F Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IEr
Plasmid#192210PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-E Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-ICr
Plasmid#192211PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus I-C Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IFl_Con-IIAr
Plasmid#192212PurposeConsensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.DepositorInsertConsensus I-F Leader and Consensus II-A Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IEl_Con-IEr
Plasmid#192213PurposeConsensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.DepositorInsertConsensus I-E Leader and Consensus I-E Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-ICl_Con-ICr
Plasmid#192214PurposeConsensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.DepositorInsertConsensus I-C Leader and Consensus I-C Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCon-IIAl_Con-IIAr
Plasmid#192215PurposeConsensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.DepositorInsertConsensus II-A Leader and Consensus II-A Locus DNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX1-IFl_Con-IFr
Plasmid#192216PurposeMutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertI-F leader and I-F repeat construct not studied further in this paper (X1)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX2-IFl_Con-IFr
Plasmid#192217PurposeMutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.DepositorInsertI-F leader and I-F repeat construct not studied further in this paper (X2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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qTAG-C-mStayGold-Puro-H2BC11-MMEJ
Plasmid#227333PurposeMMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-H2BC11
Plasmid#227330PurposeDonor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro-PLK4
Plasmid#227311PurposeDonor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)DepositorInsertPLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-PCNT
Plasmid#227285PurposeDonor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)DepositorInsertPCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxamEXP4CUP1
Plasmid#218284PurposeUse together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only